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[天然气管道防护涂层细菌分解剂胞外聚合物复合物的单糖和脂肪酸组成]

[Monosaccharide and fatty acid composition of exopolymer complex of bacteria-destructors of the protective coating of gas pipeline].

作者信息

Kopteva Zh P, Zanina V V, Boretskaia M A, Iumyna Iu M, Kopteva A E, Kozlova I A

出版信息

Mikrobiol Z. 2012 Mar-Apr;74(2):22-8.

PMID:22686014
Abstract

Monosaccharide and fatty acid composition of the exopolymer complex (EPC) of heterotrophic bacteria Pseudomonas pseudoalkaligenes 109, Pseudomonas sp. T/2, Rhodococcus erythropolis 102--destructors of the protective coating Polyken 980-25 has been studied. It is shown that qualitative and quantitative composition of EPC components changes depending on the model of bacteria growth. Arabinose, mannose, galactose and glucose are dominating saccharides. Xylose has been revealed only under conditions of the biofilm form of growth of all the studied bacteria; ribose only in the biofilm of Pseudomonas sp. T/2. The fatty acid composition of EPC contains saturated and unsaturated acids with 12-19 carbon atoms. Hexadecanoic acid (C 16:0) acid which content in the biofilm and plankton conditions is from 24.9 to 32.4% prevailed in the spectrum of fatty acids of EPC bacteria. Unsaturated fatty acids: hexadecanoic (C 16:1) and octadecenoic (C 18:1) ones have been revealed only in the biofilm of bacteria-destructors of the coating.

摘要

对嗜麦芽窄食单胞菌109、假单胞菌属T/2、红平红球菌102(均为保护涂层Polyken 980 - 25的分解菌)胞外聚合物复合物(EPC)的单糖和脂肪酸组成进行了研究。结果表明,EPC成分的定性和定量组成随细菌生长模式的不同而变化。阿拉伯糖、甘露糖、半乳糖和葡萄糖是主要糖类。仅在所有研究细菌的生物膜生长模式条件下发现了木糖;核糖仅在假单胞菌属T/2的生物膜中被发现。EPC的脂肪酸组成包含含有12 - 19个碳原子的饱和脂肪酸和不饱和脂肪酸。十六烷酸(C 16:0)在EPC细菌的脂肪酸谱中占主导地位,其在生物膜和浮游条件下的含量为24.9%至32.4%。不饱和脂肪酸:十六碳烯酸(C 16:1)和十八碳烯酸(C 18:1)仅在涂层分解菌的生物膜中被发现。

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