Institute for Prevention and Occupational Medicine of the German Social Accident Insurance (IPA), Institute of the Ruhr University Bochum, Bochum, Germany.
J Toxicol Environ Health A. 2012;75(8-10):501-7. doi: 10.1080/15287394.2012.674919.
Passive airborne dust sampling using electrostatic dustfall collectors (EDCs) is one possibility especially for long sampling periods. In this study, EDCs were deposited in living rooms of private households and in social rooms of composting plants. The aim of the study was to determine whether endotoxin and pyrogenic activity are measurable using EDCs. In all extracts, endotoxin (via Limulus amebocyte lysate [LAL] assay) and pyrogenic activity (interleukin [IL]-1β release via whole blood assay) were detectable. In addition, the monocyte chemotactic protein (MCP-1; CCL-2) as a secondary proinflammatory marker was measured with whole blood assay. Endotoxin activity and proinflammatory/pyrogenic activity of EDC extracts from social rooms in composting plants were higher compared to extracts obtained from EDCs in private household rooms. A significant correlation between LAL assay and whole blood assay was detectable. In conclusion, EDC sampling is an applicable method to evaluate settled dust from airborne bioaerosols displaying a longer period of exposure. The extraction of EDC without Tween enables one to measure endotoxin as well as proinflammatory/pyrogenic activity using the same sample for parallel detection and more reliable characterization of the airborne bioaerosol contamination.
使用静电沉降收集器（EDC）进行被动空气尘埃采样是一种可能性，尤其是对于长时间采样。在这项研究中，EDC 被沉积在私人住宅的客厅和堆肥厂的社交室中。该研究的目的是确定使用 EDC 是否可以测量内毒素和热原活性。在所有提取物中，均可检测到内毒素（通过鲎变形细胞溶解物[LAL]测定）和热原活性（通过全血测定白细胞介素[IL]-1β释放）。此外，还通过全血测定法测量单核细胞趋化蛋白（MCP-1；CCL-2）作为二级促炎标志物。与从私人住宅 EDC 获得的提取物相比，从堆肥厂社交室的 EDC 提取物中检测到的内毒素活性和促炎/热原活性更高。LAL 测定法和全血测定法之间存在显著相关性。总之，EDC 采样是一种可用于评估显示更长暴露期的空气传播生物气溶胶沉降灰尘的方法。使用不含吐温的 EDC 进行提取，可以使用相同的样品进行平行检测，从而测量内毒素以及促炎/热原活性，从而更可靠地表征空气传播生物气溶胶的污染。
