Microbiology Unit, Department of Biological Sciences, Nasarawa State University, Keffi, Nigeria.
Scand J Immunol. 2012 Jul;76(1):33-8. doi: 10.1111/j.1365-3083.2012.02700.x.
Studies on the reference values of CD4 and CD3 T cells in healthy individuals have continued to gain significance because of the importance of these immunological markers in the initiation of combination antiretroviral therapy (cART). The aim of the present study was to determine and compare the reference values of CD4 and CD3 T cells in urban and rural Nigerians who were human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV) negative. After ethical clearance and informed consent, 1123 subjects who met the inclusion criteria [mean age = 24.4 (± 11.2) years] were recruited in this study. Blood samples were analysed using the BD FACScount cytometer according to the manufacturer's instructions. Of the overall 1123 subjects, reference means of CD4, CD3 and CD4/CD3 ratio were 1030 ± 367, 1757 ± 609 cells/μl and 0.59 ± 0.08, respectively. Five hundred and fifty-one (49.1%) were an urban population with the mean CD4, CD3 and CD4/CD3 T cell ratio of 1032 ± 369, 1761 ± 612 cells/μl and 0.59 (±0.08), respectively. The remaining 572 (50.9%) were of a rural population with the mean CD4, CD3 and CD4/CD3 T cell ratio of 1028 ± 459, 1753 ± 958 cells/μl and 0.59 ± 0.13, respectively. Subjects with higher CD4 and CD3 T cells were more likely to be female than male (P < 0.05). There was no significant difference between the T cell values of the two populations (P > 0.05). Our findings provide new insight in the CD4 and CD3 T cell reference values of Nigerians.
对健康个体中 CD4 和 CD3 T 细胞参考值的研究由于这些免疫标志物在启动联合抗逆转录病毒治疗 (cART) 中的重要性而继续受到重视。本研究旨在确定和比较尼日利亚城乡 HIV、HBV 和 HCV 阴性人群中 CD4 和 CD3 T 细胞的参考值。在获得伦理批准和知情同意后，本研究共纳入 1123 名符合纳入标准的受试者[平均年龄=24.4(±11.2)岁]。根据制造商的说明，使用 BD FACScount 细胞仪分析血样。在这 1123 名受试者中，CD4、CD3 和 CD4/CD3 比值的参考平均值分别为 1030±367、1757±609 个/μl 和 0.59±0.08。其中 551 名(49.1%)为城市人口，平均 CD4、CD3 和 CD4/CD3 T 细胞比值分别为 1032±369、1761±612 个/μl 和 0.59(±0.08)。其余 572 名(50.9%)为农村人口，平均 CD4、CD3 和 CD4/CD3 T 细胞比值分别为 1028±459、1753±958 个/μl 和 0.59±0.13。CD4 和 CD3 T 细胞较高的受试者中女性多于男性(P<0.05)。两个群体的 T 细胞值之间无显著差异(P>0.05)。本研究结果为尼日利亚人的 CD4 和 CD3 T 细胞参考值提供了新的见解。
