Department of Biotechnology, Karunya University, Coimbatore, Tamil Nadu, India.
Immunopharmacol Immunotoxicol. 2012 Dec;34(6):1020-7. doi: 10.3109/08923973.2012.693086. Epub 2012 Jun 11.
All-trans retinoic acid (ATRA), an active metabolite of retinal, has been shown to exert anti-cancer activities in a number of cancer cells and tissues. Syndecan-1 is a proteoglycan, mediate cell-cell adhesion and prevent invasion in epithelial cells. The aim of the present study was to examine the level of syndecan-1 expression and the chemopreventive effect of ATRA during lung cancer development in BALB/c mice. Syndecan-1 expression was examined by immunohistochemistry using mouse monoclonal anti-human syndecan-1 antibody. In this study, benzo(α)pyrene [B(α)P] was used to induce lung cancer. The results indicated that ATRA has anti-cancer effect against B(α)P-induced lung tumor development as induced by number of tumor nodules and histopathologic report. The loss of syndecan-1 expression in the epithelial cell membrane is associated with tumor cell growth and invasiveness. Our study for syndecan-1 indicated a chemoprotective effect of ATRA against changes in lung epithelial cell membrane syndecan-1 expression in B(α)P-induced lung cancer model. Therefore ATRA could serve as effective chemotherapeutic agent against cancer invasion/metastasis, at least in the lungs.
全反式视黄酸（ATRA）是视黄醇的一种活性代谢物，已被证明在多种癌细胞和组织中具有抗癌活性。黏附素-1 是一种蛋白聚糖，介导细胞间黏附，防止上皮细胞侵袭。本研究旨在探讨黏附素-1 的表达水平以及 ATRA 在 BALB/c 小鼠肺癌发生过程中的化学预防作用。使用小鼠单克隆抗人黏附素-1 抗体通过免疫组织化学检测黏附素-1 的表达。在这项研究中，苯并（α）芘[B（α）P]用于诱导肺癌。结果表明，ATRA 对 B（α）P 诱导的肺癌肿瘤发展具有抗癌作用，表现为肿瘤结节数量和组织病理学报告。上皮细胞膜中黏附素-1 表达的丧失与肿瘤细胞的生长和侵袭性有关。我们的研究表明，ATRA 对 B（α）P 诱导的肺癌模型中肺上皮细胞膜黏附素-1 表达的变化具有化学保护作用。因此，ATRA 可作为有效的化学治疗剂，至少可用于治疗肺癌的侵袭/转移。
