十二指肠-空肠旁路术和袖状胃切除术对糖尿病 Goto-Kakizaki 大鼠 GLUT2 和葡萄糖激酶的影响及机制。

Effects and mechanism of duodenal-jejunal bypass and sleeve gastrectomy on GLUT2 and glucokinase in diabetic Goto-Kakizaki rats.

机构信息

Department of Gastrointestinal Surgery, Shanghai Tenth People's Hospital, Shanghai Tongji University, #301 Yanchang Zhong Rd, Shanghai, 200073, China.

出版信息

Eur J Med Res. 2012 Jun 11;17(1):15. doi: 10.1186/2047-783X-17-15.

BACKGROUND

The study investigated the effects and mechanism of duodenal-jejunal bypass (DJB) and sleeve gastrectomy (SG) on the expression of liver GLUT2 and glucokinase (GCK) in diabetic rats.

METHODS

Animal models of Goto-Kakizaki (GK) rats were established for the investigation of DJB and SG. Results of weight, food intake, fasting plasma glucose level, oral glucose tolerance test and insulin were compared. Liver tissues were harvested 8 weeks postoperatively. Reverse transcription-PCR and western blot were used to detect liver GLUT2 and GCK mRNA and protein expression after operation.

RESULTS

Fasting plasma glucose levels of DJB group and SG group in GK rats were markedly declined at 3 days and l, 2, 4, 6, and 8 weeks postoperatively (P <0.01), whereas the levels of the sham-operated group only dropped at 3 days and 1 week postoperatively, and there were no significant differences 2 weeks postoperatively (P >0.05). In the liver of GK rats, GLUT2 mRNA level and protein expression after DJB were higher than those in sham-operated group and control group. GLUT2 mRNA level and protein expression after SG were significantly lower than those in control group (P <0.01). GCK mRNA and protein experienced similar expression change.

CONCLUSIONS

Both DJB and SG can decrease the plasma glucose levels of GK rats, whereas they have different effects on the expression of liver GLUT2 and GCK.

背景

本研究旨在探讨十二指肠空肠旁路术（DJB）和袖状胃切除术（SG）对糖尿病大鼠肝脏葡萄糖转运蛋白 2（GLUT2）和葡萄糖激酶（GCK）表达的影响及其机制。

方法

建立 Goto-Kakizaki（GK）大鼠动物模型，用于 DJB 和 SG 的研究。比较体重、摄食量、空腹血糖水平、口服葡萄糖耐量试验和胰岛素结果。术后 8 周采集肝组织。采用反转录-聚合酶链反应和蛋白质印迹法检测术后肝脏 GLUT2 和 GCK mRNA 和蛋白表达。

结果

GK 大鼠 DJB 组和 SG 组术后 3 天、1、2、4、6 和 8 周空腹血糖水平明显下降（P<0.01），而假手术组仅在术后 3 天和 1 周下降，术后 2 周无明显差异（P>0.05）。在 GK 大鼠肝脏中，DJB 后 GLUT2 mRNA 水平和蛋白表达高于 sham 组和对照组。SG 后 GLUT2 mRNA 水平和蛋白表达明显低于对照组（P<0.01）。GCK mRNA 和蛋白表达也发生了相似的变化。