Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria, 3122, Australia.
Biofouling. 2012;28(6):539-50. doi: 10.1080/08927014.2012.694426.
Superhydrophobic titanium surfaces fabricated by femtosecond laser ablation to mimic the structure of lotus leaves were assessed for their ability to retain coccoid bacteria. Staphylococcus aureus CIP 65.8T, S. aureus ATCC 25923, S. epidermidis ATCC 14990T and Planococcus maritimus KMM 3738 were retained by the surface, to varying degrees. However, each strain was found to preferentially attach to the crevices located between the microscale surface features. The upper regions of the microscale features remained essentially cell-free. It was hypothesised that air entrapped by the topographical features inhibited contact between the cells and the titanium substratum. Synchrotron SAXS revealed that even after immersion for 50 min, nano-sized air bubbles covered 45% of the titanium surface. After 1 h the number of cells of S. aureus CIP 65.8T attached to the lotus-like titanium increased to 1.27×10(5) mm(-2), coinciding with the replacement of trapped air by the incubation medium.
通过飞秒激光烧蚀来模拟荷叶结构而制造的超疏水钛表面，评估其保留球菌的能力。不同程度地，金黄色葡萄球菌 CIP 65.8T、金黄色葡萄球菌 ATCC 25923、表皮葡萄球菌 ATCC 14990T 和海洋节杆菌 KMM 3738 被保留在表面上。然而，每种菌株都被发现优先附着在微尺度表面特征之间的缝隙上。微尺度特征的上部区域基本上保持无细胞状态。据推测，拓扑特征截留的空气抑制了细胞与钛基底之间的接触。同步加速器小角 X 射线散射 (SAXS) 显示，即使在浸泡 50 分钟后，纳米大小的气泡仍覆盖了 45%的钛表面。1 小时后，附着在类荷叶钛表面的金黄色葡萄球菌 CIP 65.8T 的细胞数量增加到 1.27×10(5)mm(-2)，这与被孵育介质取代截留空气的时间相对应。
