Esteban David, Parker Scott, Schriewer Jill, Hartzler Hollyce, Buller R Mark
Biology Department, Vassar College, Poughkeepsie, NY, USA.
Methods Mol Biol. 2012;890:177-98. doi: 10.1007/978-1-61779-876-4_11.
Ectromelia virus infections in the laboratory mouse have emerged as a valuable model to investigate human orthopoxvirus infections to understand the progression of disease, to discover and characterize antiviral treatments, and to study the host-pathogen relationship as it relates to pathogenesis and the immune response. Here we describe how to safely work with the virus and protocols for common procedures for the study of ectromelia virus in the laboratory mouse including the preparation of virus stocks, the use of various routes of inoculation, and collection of blood and tissue from infected animals. In addition, several procedures are described for assessing the host response to infection: for example, measurement of virus-specific CD8 T cells and the use of ELISA and neutralization assays to measure orthopoxvirus-specific antibody titers.
实验室小鼠中的痘苗病毒感染已成为一种有价值的模型，用于研究人类正痘病毒感染，以了解疾病的进展，发现和表征抗病毒治疗方法，并研究与发病机制和免疫反应相关的宿主-病原体关系。在此，我们描述了如何安全地处理该病毒以及在实验室小鼠中研究痘苗病毒的常见程序方案，包括病毒储备液的制备、各种接种途径的使用以及从感染动物采集血液和组织。此外，还描述了几种评估宿主对感染反应的程序：例如，测量病毒特异性CD8 T细胞以及使用ELISA和中和试验来测量正痘病毒特异性抗体滴度。
