Meng Xiangzhi, Xiang Yan
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Methods Mol Biol. 2012;890:219-32. doi: 10.1007/978-1-61779-876-4_13.
Monoclonal antibodies to specific vaccinia virus (VACV) proteins are valuable reagents in studies of VACV. In this chapter, we describe methods of generating a panel of monoclonal antibodies that recognize a variety of VACV proteins in their native conformation in infected cells. The antibodies thus generated recognize mostly VACV proteins that are involved in virion assembly or/and are major antigens in smallpox vaccine. These antibodies are useful for tracking distinct steps in virion assembly and for studying the B cell epitopes in smallpox vaccine.
针对特定痘苗病毒（VACV）蛋白的单克隆抗体是痘苗病毒研究中的宝贵试剂。在本章中，我们描述了产生一组单克隆抗体的方法，这些抗体能够识别感染细胞中天然构象的多种痘苗病毒蛋白。由此产生的抗体主要识别参与病毒粒子组装或/且是天花疫苗主要抗原的痘苗病毒蛋白。这些抗体可用于追踪病毒粒子组装的不同步骤以及研究天花疫苗中的B细胞表位。
