生成和鉴定针对天花病毒的大量鼠源单克隆抗体。
Generation and characterization of a large panel of murine monoclonal antibodies against vaccinia virus.
机构信息
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
出版信息
Virology. 2011 Jan 20;409(2):271-9. doi: 10.1016/j.virol.2010.10.019. Epub 2010 Nov 5.
Vaccinia virus (VACV), the vaccine for smallpox, induces an antibody response that is largely responsible for conferring protection. Here, we studied the antibody response to VACV by generating and characterizing B cell hybridomas from a mouse immunized with VACV. Antibodies from 66 hybridomas were found to recognize 11 VACV antigens (D8, A14, WR148, D13, H3, A56, A33, C3, B5, A10 and F13), 10 of which were previously recognized as major antigens in smallpox vaccine by a microarray of VACV proteins produced with a prokaryotic expression system. VACV C3 protein, which was not detected as a target of antibody response by the proteome array, was recognized by two hybridomas, suggesting that selection of hybridomas based on immune recognition of infected cells has the advantage of detecting additional antibody response to native VACV antigens. In addition, these monoclonal antibodies are valuable reagents for studying poxvirus biology and protective mechanism of smallpox vaccine.
痘苗病毒（VACV）是天花疫苗，它诱导的抗体反应在很大程度上负责提供保护。在这里，我们通过从接种 VACV 的小鼠中生成和鉴定 B 细胞杂交瘤来研究针对 VACV 的抗体反应。从 66 个杂交瘤中分离出的抗体识别了 11 种 VACV 抗原（D8、A14、WR148、D13、H3、A56、A33、C3、B5、A10 和 F13），其中 10 种先前被微阵列识别为天花疫苗中的主要抗原通过用原核表达系统产生的 VACV 蛋白的微阵列。VACV C3 蛋白未被蛋白质组阵列检测为抗体反应的靶标，但被两个杂交瘤识别，这表明基于感染细胞的免疫识别选择杂交瘤具有检测针对天然 VACV 抗原的额外抗体反应的优势。此外，这些单克隆抗体是研究痘病毒生物学和天花疫苗保护机制的有价值的试剂。
Zotero 插件