Hu J G, Yokoyama T, Kitagawa T
Faculty of Pharmaceutical Sciences, Nagasaki University, Japan.
Chem Pharm Bull (Tokyo). 1990 Jul;38(7):1961-5. doi: 10.1248/cpb.38.1961.
The effects of several conditions for the immunization of mice was studied using an aliquot of a viomycin (VM) protein conjugate as the common primary or booster antigen. Responses of the mice were assessed by measuring mouse serum levels of total immunoglobulin G (IgG) and anti-VM antibody responses using the newly improved two assay methods. The choice of route was found to be a very important factor in immunization and intraperitoneal injection was the most optimal among the four routes studied. The effect of the concentration of Mycobacteria in Freund's complete adjuvant (FCA) was also studied, and it was found that a diluted FCA was more effective than a commercial FCA. The effect of the controlled release of the antigen was studied and three important phenomena were observed: The mice immunized by the mini-osmotic pump-aided controlled release of the antigen responded with similar small amounts of both total IgG and anti-VM antibody regardless of the presence or absence of FCA in the antigen; emulsifying the antigen with FCA was a very important condition for the effective elicitation of the specific antibody; a mixture of antigen and FCA without emulsifying produced little specific antibody and a large amount of total IgG. The more effectively immunized mice responded with a larger decrease in body weight soon after the primary injection.
使用硫酸链霉素（VM）蛋白偶联物的一份子作为共同的初次或加强抗原，研究了几种小鼠免疫条件的效果。通过使用新改进的两种检测方法测量小鼠血清中总免疫球蛋白G（IgG）水平和抗VM抗体反应，来评估小鼠的反应。发现免疫途径的选择是免疫中的一个非常重要的因素，在所研究的四种途径中，腹腔注射是最理想的。还研究了弗氏完全佐剂（FCA）中分枝杆菌浓度的影响，发现稀释的FCA比市售FCA更有效。研究了抗原控释的效果，观察到三个重要现象：无论抗原中是否存在FCA，通过微型渗透泵辅助控释抗原免疫的小鼠，其总IgG和抗VM抗体的反应量都相似且较少；用FCA乳化抗原是有效诱导特异性抗体的一个非常重要的条件；未乳化的抗原和FCA混合物产生的特异性抗体很少，而总IgG量很大。免疫效果更好的小鼠在初次注射后不久体重下降幅度更大。
