Hu J G, Ide A, Yokoyama T, Kitagawa T
Chem Pharm Bull (Tokyo). 1989 Nov;37(11):3042-6. doi: 10.1248/cpb.37.3042.
This study was undertaken to establish the optimal immunogen dose for immunization of mice, using a viomycin-protein conjugate as a hapten immunogen. It was found that specific immunoglobulin G (IgG) formation depends on both the dose of antigen and the type of adjuvant: the optimal antigen dose for an immune response is quite different depending on whether the mice are being treated with Freund's complete adjuvant (FCA) or Freund's incomplete adjuvant (FICA). The total IgG amount depends mainly upon the type of adjuvant used. FCA gave the double the level of IgG compared to that obtained with FICA. The antigen dose was found to have little influence on the total production of IgG. Mice given a primary immunization with 10 micrograms of antigen emulsified in FCA and then given a booster with the same amount of antigen emulsified in FICA produced a strikingly high level of specific anti-viomycin antibody of over 2.5 mg/ml of the antiserum. It was also found that decreases in the weight of the mice were related to the kind of adjuvant used as well as to the level of the specific antibody formed.
本研究旨在使用紫霉素 - 蛋白质结合物作为半抗原免疫原，确定小鼠免疫的最佳免疫原剂量。结果发现，特异性免疫球蛋白G（IgG）的形成取决于抗原剂量和佐剂类型：根据小鼠是用弗氏完全佐剂（FCA）还是弗氏不完全佐剂（FICA）处理，免疫反应的最佳抗原剂量差异很大。总IgG量主要取决于所用佐剂的类型。与FICA相比，FCA产生的IgG水平高出一倍。发现抗原剂量对IgG的总产量影响很小。用10微克抗原乳化于FCA中进行初次免疫，然后用相同量抗原乳化于FICA中进行加强免疫的小鼠，产生了超过2.5毫克/毫升抗血清的极高水平的特异性抗紫霉素抗体。还发现小鼠体重的减轻与所用佐剂的种类以及形成的特异性抗体水平有关。
