Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, CE, Brazil.
Pest Manag Sci. 2012 Oct;68(10):1380-4. doi: 10.1002/ps.3316. Epub 2012 Jun 12.
Myracrodruon urundeuva Fr. Allemao is a common tree in the Caatinga that has been widely used for various medical purposes. Previous studies showed that the ethanol seed extract of M. urundeuva has potent activity against the larval stage of the dengue vector Aedes aegypti. Given this potential insecticidal activity, bioguided separation steps were performed in order to isolate the active compound(s).
The isolation process resulted in only one active chemical compound, identified by infrared spectroscopy and mass spectrometry as m-pentadecadienyl-phenol. This compound presented potent larvicidal and pupicidal activity (LC50 10.16 and 99.06 µg mL(-1) respectively) and great egg hatching inhibitory activity (IC50 49.79 µg mL(-1)). The mode of action was investigated through observations of behavioural and morphological changes performed in third-instar larvae treated with m-pentadecadienyl-phenol solution after 1, 6, 12, 16 and 20 h of exposure. Some changes were observed as flooding of the tracheal system, alterations in siphonal valves and anal gills and lethargy, probably caused by the strong anticholinesterasic activity reported previously.
The compound isolated from M. urundeuva seeds, m-pentadecadienyl-phenol, showed potent activity against immature stages of dengue vector, Ae. aegypti, being considered the main larvicidal principle.
Myracrodruon urundeuva Fr. Allemao 是一种常见的 Caatinga 树种，已被广泛用于各种医疗用途。先前的研究表明，M. urundeuva 的乙醇种子提取物对登革热传播媒介埃及伊蚊的幼虫期具有很强的活性。鉴于这种潜在的杀虫活性，进行了生物导向分离步骤以分离活性化合物。
分离过程仅得到一种活性化学化合物，通过红外光谱和质谱鉴定为 m-十五碳二烯基-苯酚。该化合物表现出很强的杀幼虫和杀蛹活性（LC50 分别为 10.16 和 99.06 µg mL(-1)）和很强的卵孵化抑制活性（IC50 为 49.79 µg mL(-1)）。通过观察在暴露后 1、6、12、16 和 20 小时用 m-十五碳二烯基-苯酚溶液处理的三龄幼虫的行为和形态变化，研究了作用方式。观察到一些变化，如气管系统充水、虹吸管瓣膜和肛门鳃的改变以及昏睡，这可能是由先前报道的强烈抗胆碱酯酶活性引起的。
从 M. urundeuva 种子中分离出的化合物 m-十五碳二烯基-苯酚对登革热传播媒介埃及伊蚊的幼体表现出很强的活性，被认为是主要的杀幼虫剂。
