Serdeiro Michele Teixeira, Dias Thiago Dutra, de Lima Natanael Teles Ramos, Barbosa-Filho José Maria, Belato Renato de Souza, Santos-Mallet Jacenir Reis Dos, Maleck Marise
Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil.
Laboratório de Insetos Vetores, Campus Vassouras and Campus Maricá, Universidade de Vassouras, Vassouras 27700-000, Brazil.
Trop Med Infect Dis. 2023 Sep 7;8(9):440. doi: 10.3390/tropicalmed8090440.
Dengue, Chikungunya and Zika are arboviruses, transmitted by the mosquito , that cause high mortality and serious health consequences in human populations. Efforts to control are important for preventing outbreaks of these diseases. Essential oil constituents are known to exhibit many activities, such as their use as larvicides. Given their potential, the present study aimed to characterize the larvicidal effect of dihydrojasmone, p-cymene, carvacrol, thymol, farnesol and nerolidol on the larvae of and their interference over the morphology of the mosquitos. The essential oil constituents were dissolved in dimethylsulfoxide at concentrations of 1-100 μg/mL and were applied in the breeding environment of third-stage larvae. The larvae from bioassays were fixed, dehydrated and embedded. Ultrathin sections were contrasted using 5% uranyl acetate and 1% lead citrate for observation through transmission electron microscopy. The oil with the highest larvicidal efficiency was found to be nerolidol, followed by farnesol, p-cymene, carvacrol, thymol and dihydrojasmone, with an LC of 11, 21, 23, 40, 45 and 66 µg/mL, respectively. The treated larvae caused alteration to the tegument or internal portions of larvae. The present study demonstrated which of these oils-dihydrojasmone, farnesol, thymol, p-cymene, carvacrol and nerolidol-have effective larvicidal activity.
登革热、基孔肯雅热和寨卡病毒是虫媒病毒，通过蚊子传播，可导致人类高死亡率和严重的健康后果。控制这些疾病的传播对于预防疫情爆发至关重要。已知精油成分具有多种活性，例如用作杀幼虫剂。鉴于其潜力，本研究旨在表征二氢茉莉酮、对伞花烃、香芹酚、百里酚、法尼醇和橙花叔醇对埃及伊蚊幼虫的杀幼虫作用及其对蚊子形态的影响。将精油成分溶解于二甲基亚砜中，浓度为1-100μg/mL，并应用于三期幼虫的滋生环境中。生物测定中的幼虫经过固定、脱水和包埋处理。超薄切片用5%醋酸铀和1%柠檬酸铅进行对比，以便通过透射电子显微镜进行观察。发现杀幼虫效率最高的油是橙花叔醇，其次是法尼醇、对伞花烃、香芹酚、百里酚和二氢茉莉酮，其LC50分别为11、21、23、40、45和66μg/mL。经处理的埃及伊蚊幼虫导致幼虫体表或内部发生变化。本研究证明了这些油中二氢茉莉酮、法尼醇、百里酚、对伞花烃、香芹酚和橙花叔醇具有有效的杀幼虫活性。
