School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Evid Based Complement Alternat Med. 2012;2012:701289. doi: 10.1155/2012/701289. Epub 2012 May 29.
The purpose of this study was to determine whether compound zhi zhu xiang (CZZX) exerts anxiolytic-like effects in rats. The animals were orally administered CZZX (0.75, 1.5, and 3 g/kg daily) for 10 days and tested in the elevated plus maze (EPM), Vogel conflict test (VCT), and open field. Repeated treatment with CZZX (3 g/kg/day, p.o.) significantly increased the percentage of both entries into and time spent on the open arms of the EPM compared with saline controls. In the VCT, repeated treatment with CZZX (1.5 and 3 g/kg/day, p.o.) significantly increased the number of punished licks. The drug did not change the total entries into the open arms of the EPM or interfere with water consumption or nociceptive threshold, discarding potential confounding factors in the two tests. In the open field, locomotion was not reduced, discarding the possible sedative effect of CZZX. In the binding assay, the binding of [(3)H] Ro 15-1788 (flumazenil) to the benzodiazepine binding site in washed crude synaptosomal membranes from rat cerebral cortex was affected by CZZX. These data indicate an anxiolytic-like profile of action for CZZX without sedative side effects, and this activity may be mediated by benzodiazepine binding site modulation at γ-aminobutyric acid-A receptors.
本研究旨在探讨枳术香（CZZX）是否对大鼠具有抗焦虑样作用。动物连续 10 天经口给予 CZZX（0.75、1.5 和 3 g/kg），并在高架十字迷宫（EPM）、Vogel 冲突试验（VCT）和旷场试验中进行测试。重复给予 CZZX（3 g/kg/天，po）可显著增加 EPM 开臂的进入次数和停留时间百分比，与生理盐水对照组相比。在 VCT 中，重复给予 CZZX（1.5 和 3 g/kg/天，po）可显著增加受罚舔次数。该药物未改变 EPM 开臂的总进入次数，也不影响水摄入量或痛觉阈值，排除了这两个试验中可能存在的混杂因素。在旷场试验中，运动行为未减少，排除了 CZZX 可能具有的镇静作用。在结合测定中，CZZX 影响了 [(3)H] Ro 15-1788（氟马西尼）与大鼠大脑皮质粗突触体膜上苯二氮䓬结合部位的结合。这些数据表明 CZZX 具有抗焦虑样作用而无镇静副作用，这种活性可能通过γ-氨基丁酸-A 受体苯二氮䓬结合部位的调节来介导。
