Stiazhkina E V, Obvintseva N A, Shaposhnikova I A, Triapitsyna G A, Stukalov P M, Priakhin E A
Radiats Biol Radioecol. 2012 Mar-Apr;52(2):198-206.
The Comet Assay and micronucleus assays have been used to evaluate the condition of the nuclear DNA in erythrocytes of peripheral blood of roach (Rutilus rutilus L.) from water-storage of low-level radioactive waste. The Rutilus rutilus L. from the Shershny reservoir, Chelyabinsk, was used as a control population. Radionuclide maintenance in water, sediments and roach in those reservoirs and Shershny reservoir was defined. The dose rate for Rutilus rutilus L. was calculated using program complex ERICA Assessment Tool 1.0 May 2009. Our investigation has shown that a chronic radiation of population (dose rate - 5.2 mGy/day and 19.5 mGy/day) leads to a significantly higher level of the DNA damage in erythrocytes of peripheral blood and increases the speed of nuclear DNA reparation after irradiation of erythrocytes in vitro. We suppose that it may be a result of the increased quantity of active form of oxygen in cells of the fish in water-storage of low-level radioactive waste.
彗星试验和微核试验已被用于评估来自低水平放射性废物储存水体中的拟鲤（Rutilus rutilus L.）外周血红细胞中核DNA的状况。以车里雅宾斯克州舍尔什尼水库的拟鲤作为对照群体。测定了这些水库以及舍尔什尼水库水体、沉积物和拟鲤中的放射性核素含量。使用2009年5月的ERICA评估工具1.0程序组合计算了拟鲤的剂量率。我们的研究表明，群体的慢性辐射（剂量率分别为5.2毫戈瑞/天和19.5毫戈瑞/天）会导致外周血红细胞中DNA损伤水平显著升高，并提高红细胞体外照射后核DNA的修复速度。我们推测，这可能是低水平放射性废物储存水体中鱼类细胞内活性氧数量增加的结果。
