Department of Medical, Surgical and Health Sciences, University of Trieste, Italy.
Angle Orthod. 2012 Nov;82(6):1047-52. doi: 10.2319/123111-806.1. Epub 2012 Mar 26.
To evaluate gingival crevicular fluid (GCF) protein content and alkaline phosphatase (ALP) activity in growing subjects in relation to stages of skeletal maturation, ie, the growth phase, as prepubertal, pubertal, and postpubertal.
Fifty healthy growing subjects (31 girls and 19 boys; age range, 7.8-17.7 years) were enrolled in this study that followed a double-blind, prospective, cross-sectional design. Collection of GCF was performed at the mesial and distal sites of both central incisors, for the maxilla and mandible. Growth phase was assessed through the cervical vertebral maturation method. GCF parameters were expressed as total protein content, total ALP activity, and normalized ALP activity.
The total GCF protein content was similar between the different growth phases. On the contrary, the total ALP activity showed a peak for the pubertal growth phase. The normalized GCF ALP activity was only poorly associated with growth phase. No differences were seen between the maxillary and mandibular sites, or between the sexes, for any GCF parameter.
The total GCF protein content is not sensitive to the growth phase. However, GCF ALP activity has potential as a diagnostic aid for identification of the pubertal growth phase in individual subjects when expressed as total, but not normalized, values.
评估生长发育期龈沟液（GCF）蛋白含量和碱性磷酸酶（ALP）活性与骨骼成熟阶段的关系，即生长阶段，包括青春前期、青春期和青春后期。
本研究采用双盲、前瞻性、横断面设计，共纳入 50 名健康生长的受试者（31 名女孩和 19 名男孩；年龄 7.8-17.7 岁）。在上下中切牙的近中和远中位点采集 GCF。通过颈椎成熟度评估生长阶段。GCF 参数表示为总蛋白含量、总 ALP 活性和标准化 ALP 活性。
不同生长阶段的总 GCF 蛋白含量相似。相反，总 ALP 活性在青春期生长阶段达到峰值。标准化 GCF ALP 活性与生长阶段的相关性较差。任何 GCF 参数在上颌和下颌部位或性别之间均无差异。
总 GCF 蛋白含量对生长阶段不敏感。然而，当以总而非标准化值表示时，GCF ALP 活性具有作为个体青春期生长阶段识别的诊断辅助工具的潜力。
