Liu Ya-li, Bi Hui, Fan Rong, Li Yu-heng, Wang Yue-min, Chen Yao-ming, Chen Jing-yuan, Chi Su-min, Pei Jian-ming
Department of Physiology, Fourth Military Medical University, Xi'an, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Jun;28(6):601-3.
To investigate the effect of compound nutrients on Th1/Th2 imbalance caused by changes in cytokines of Th cell subsets, interleukin (IL)-2, IL-6 and TNF-α, in rats with acute immobilization and cold water-immersion stress.
Male SD rats were randomly assigned to three groups including normal control group (C), acute stress group (S) and acute stress+compound nutrients group (S+CN). Stress procedure was the acute immobilization and cold water-immersion. The stress rats were fed water (Group S) or compound nutrient liquid (Group S+CN) by a feeding needle 1 week before acute stress, and then restrained and immersed in cold water for 30 min. The control rats were given water in the same way without stress stimulation. The rats were killed and blood samples were collected 0, 30, 60 and 120 min after stress, respectively. Serum was separated by centrifugation and stored at -70 DegreesCelsius until assayed. The serum levels of IL-2, IL-6 and TNF-α were analyzed by an enzyme-linked immunosorbent assay (ELISA) kit.
Acute immobilization and cold water-immersion stress reduced IL-2 level, and increased IL-6 and TNF-α level at different time points (0, 30, 60 and 120 min) after stress, which was most obvious at 30 min. Oral administration (gavage) of compound nutrients was found to moderate the acute immobilization and cold water-immersion stress-induced changes in serum IL-2, IL-6 and TNF-α, which was also most significant at 30 min after stress.
Complex nutrients can significantly alleviate the changes of Th1/Th2 cytokines in stress rats, including IL-2, IL-6 and TNF-α, which suggests that compound nutrients can improve the immune regulation function of stress rats and restore Th1/Th2 balance. Compound nutrients might enhance the body's anti-stress ability and lighten the stress-related damage, thus being a possible candidate for the therapeutic modulation of stress.
研究复合营养素对急性制动和冷水浸泡应激大鼠因Th细胞亚群细胞因子白细胞介素（IL）-2、IL-6和肿瘤坏死因子-α变化所致Th1/Th2失衡的影响。
雄性SD大鼠随机分为三组，包括正常对照组（C）、急性应激组（S）和急性应激+复合营养素组（S+CN）。应激程序为急性制动和冷水浸泡。应激大鼠在急性应激前1周通过饲管给予水（S组）或复合营养液（S+CN组），然后束缚并浸入冷水中30分钟。对照大鼠以相同方式给予水但无应激刺激。分别在应激后0、30、60和120分钟处死大鼠并采集血样。通过离心分离血清并储存于-70℃直至检测。采用酶联免疫吸附测定（ELISA）试剂盒分析血清IL-2、IL-6和TNF-α水平。
急性制动和冷水浸泡应激降低了应激后不同时间点（0、30、60和120分钟）的IL-2水平，并升高了IL-6和TNF-α水平，在30分钟时最为明显。发现口服（灌胃）复合营养素可缓解急性制动和冷水浸泡应激诱导的血清IL-2、IL-6和TNF-α变化，在应激后30分钟时也最为显著。
复合营养素可显著减轻应激大鼠Th1/Th2细胞因子的变化，包括IL-2、IL-6和TNF-α，这表明复合营养素可改善应激大鼠的免疫调节功能并恢复Th1/Th2平衡。复合营养素可能增强机体的抗应激能力并减轻应激相关损伤，因此可能是应激治疗调节的候选药物。
