Vozzi Giovanni, Tirella Annalisa, Ahluwalia Arti
Centro E. Piaggio, Faculty of Engineering, University of Pisa, Pisa, Italy.
Methods Mol Biol. 2012;868:57-69. doi: 10.1007/978-1-61779-764-4_4.
To create composite synthetic scaffolds with the same degree of complexity and multilevel organization as biological tissue, we need to integrate multilevel biomaterial processing in rapid prototyping systems. The scaffolds then encompass the entire range of properties, which characterize biological tissue. A multilevel microfabrication system, PAM(2), has been developed to address this gap in material processing. It is equipped with different modules, each covering a range of material properties and spatial resolutions. Together, the modules in PAM(2) can be used to realize complex and composite scaffolds for tissue engineering, bringing us a step closer to real clinical applications. This chapter describes the PAM(2) system and discusses some of the practical issues associated with scaffold microfabrication and biomaterial processing.
为了创建具有与生物组织相同程度的复杂性和多级组织结构的复合合成支架，我们需要在快速成型系统中整合多级生物材料加工。这样的支架随后涵盖了表征生物组织的所有特性范围。已开发出一种多级微制造系统PAM(2)来弥补材料加工方面的这一差距。它配备了不同的模块，每个模块涵盖一系列材料特性和空间分辨率。PAM(2)中的这些模块一起可用于实现用于组织工程的复杂复合支架，使我们离实际临床应用更近一步。本章介绍了PAM(2)系统，并讨论了与支架微制造和生物材料加工相关的一些实际问题。
