Key Laboratory of Colloid and Interface Chemistry, Shandong University , Ministry of Education, Jinan, 250100, P. R. China.
ACS Appl Mater Interfaces. 2012 Jun 27;4(6):3278-84. doi: 10.1021/am300634n. Epub 2012 Jun 14.
A globular protein, β-lactoglobulin (BLG), was used to decorate reduced graphene oxide sheets (RGO) and the obtained BLG-RGO composite can be dispersed in aqueous solution with pH-sensitive solubility. The morphology of the BLG-RGO composite was studied by transmission electron microscopy (TEM) and atomic force microscopy (AFM). The results indicate that BLG-RGO is effectively exfoliated with an average thickness of 2.5 nm. UV-vis spectra were performed to examine the reduction degree and determine the optimum concentration of β-lactoglobulin and appropriate pH value. Furthermore, Raman spectra demonstrate that β-Lactoglobulin promotes the chemical reduction process of graphene oxide and benefits to repair the crystal defects. Due to the adsorption of β-Lactoglobulin on the surface of graphene sheets, the BLG-RGO composite was further used as template for Au nanoparticles assembly. These Au nanoparticles assembled on the BLG-RGO composite were shown to yield a large SERS enhancement for Rhodamine 6G.
一种球形蛋白质，β-乳球蛋白（BLG），被用来修饰还原氧化石墨烯片（RGO），所得的 BLG-RGO 复合材料可以在 pH 值敏感的水溶液中分散，具有溶解度。通过透射电子显微镜（TEM）和原子力显微镜（AFM）研究了 BLG-RGO 复合材料的形态。结果表明，BLG-RGO 被有效地剥离，平均厚度为 2.5nm。紫外可见光谱用于检查还原程度，并确定β-乳球蛋白的最佳浓度和合适的 pH 值。此外，拉曼光谱表明β-乳球蛋白促进了氧化石墨烯的化学还原过程，有利于修复晶体缺陷。由于β-乳球蛋白吸附在石墨烯片的表面上，BLG-RGO 复合材料进一步用作金纳米粒子组装的模板。在 BLG-RGO 复合材料上组装的这些金纳米粒子对罗丹明 6G 表现出很大的 SERS 增强效果。
