大鼠肝脏线粒体中的三羧酸载体。纯化、重组及动力学特性研究。
The tricarboxylate carrier from rat liver mitochondria. Purification, reconstitution and kinetic characterization.
作者信息
Glerum D M, Claeys D, Mertens W, Azzi A
机构信息
Institut für Biochemie and Molekularbiologie, Universität Bern, Switzerland.
出版信息
Eur J Biochem. 1990 Dec 12;194(2):681-4. doi: 10.1111/j.1432-1033.1990.tb15668.x.
The tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A Km of 40 microM and a Vmax of 1.56 mumol x min-1 x mg-1 have been determined for the carrier. Cholesterol levels of between 5-10% of total lipid content are shown to cause a decrease in carrier activity.
大鼠肝脏线粒体中的三羧酸载体已被纯化并重新组装到磷脂囊泡中。其活性已通过放射性柠檬酸盐摄取测定法和偶联酶测定法进行了表征。已确定该载体的 Km 为 40 μM，Vmax 为 1.56 μmol·min⁻¹·mg⁻¹。结果表明，胆固醇水平在总脂质含量的 5%至 10%之间会导致载体活性降低。
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