Department of Cardiology, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China.
J Lipid Res. 2012 Sep;53(9):1952-7. doi: 10.1194/jlr.P023028. Epub 2012 Jun 12.
Apolipoprotein (apo) O is a novel apolipoprotein that is present predominantly in high density lipoprotein (HDL). However, overexpression of apoO does not impact on plasma HDL levels or functionality in human apoA-I transgenic mice. Thus, the physiological function of apoO is not yet known. In the present study, we investigated relationships between plasma apoO levels and high-sensitive C-reactive protein (hs-CRP) levels, as well as other lipid parameters in healthy subjects (n = 111) and patients with established acute coronary syndrome (ACS) (n = 50). ApoO was measured by the sandwich dot-blot technique with recombinant apoO as a protein standard. Mean apoO level in healthy subjects was 2.21 ± 0.83 µg/ml whereas it was 4.94 ± 1.59 µg/ml in ACS patients. There were significant differences in plasma level of apoO between two groups (P < 0.001). In univariate analysis, apoO correlated significantly with lg(hsCRP) (r = 0.48, P < 0.001) in ACS patients. Notably, no significant correlation between apoO and other lipid parameters was observed. Logistic regression analysis showed that plasma apoO level was an independent predictor of ACS (OR = 5.61, 95% CI 2.16-14.60, P < 0.001). In conclusion, apoO increased in ACS patients, and may be regarded as an independent inflammatory predictor of ACS patients.
载脂蛋白 O 是一种新型载脂蛋白，主要存在于高密度脂蛋白（HDL）中。然而，在人载脂蛋白 AI 转基因小鼠中，apoO 的过表达并不影响血浆 HDL 水平或功能。因此，apoO 的生理功能尚不清楚。在本研究中，我们研究了健康受试者（n = 111）和确诊的急性冠脉综合征（ACS）患者（n = 50）的血浆 apoO 水平与高敏 C 反应蛋白（hs-CRP）水平以及其他脂质参数之间的关系。apoO 采用重组 apoO 作为蛋白标准的夹心斑点印迹技术进行测量。健康受试者的平均 apoO 水平为 2.21 ± 0.83 µg/ml，而 ACS 患者的平均 apoO 水平为 4.94 ± 1.59 µg/ml。两组间 apoO 血浆水平存在显著差异（P < 0.001）。在单因素分析中，ACS 患者的 apoO 与 lg(hsCRP)显著相关（r = 0.48，P < 0.001）。值得注意的是，apoO 与其他脂质参数之间无显著相关性。Logistic 回归分析显示，血浆 apoO 水平是 ACS 的独立预测因子（OR = 5.61，95% CI 2.16-14.60，P < 0.001）。综上所述，ACS 患者的 apoO 增加，可能被视为 ACS 患者的独立炎症预测因子。
