Chigorno V, Valsecchi M, Sonnino S, Tettamanti G
Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.
FEBS Lett. 1990 Dec 17;277(1-2):164-6. doi: 10.1016/0014-5793(90)80834-6.
GM1 ganglioside tritium-labeled at C-3 of sphingosine has been administered to rat cerebellar granule cells. Tritiated polysialylated gangliosides were observed in the cytosol of the cells, where they resulted in a higher amount after a short period of chase. This, together with the data showing an increase of the tritiated polysialylated gangliosides in the total particulate fraction in parallel to the prolonging of the chase period, suggests that cytosolic gangliosides could be a way of transporting neosynthesized gangliosides from the Golgi apparatus to the plasma membranes.
