Valsecchi M, Chigorno V, Sonnino S, Tettamanti G
Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.
Chem Phys Lipids. 1992 Jan-Feb;60(3):247-52. doi: 10.1016/0009-3084(92)90076-2.
A study has been made of the association properties of the two GM1 ganglioside molecular species GM1-C18 and GM1-C20 (containing C18 and C20 long chain bases, respectively) to rat cerebellar granule cells in culture. Both gangliosides recognized, to the same extent, and associated with them to give a form of association, the trypsin-labile form. This form was removed by treatment with trypsin enzyme. Both gangliosides associated stably with the cells to become components of the cell membranes. Although similar amounts of the two gangliosides entered the cells, being then metabolized, the time course of the association was different for the two gangliosides: after 15 h of ganglioside-cell incubation the amount of GM1-C18 inserted into the cell membrane was 2.43 times higher than that of GM1-C20.
对两种GM1神经节苷脂分子种类GM1 - C18和GM1 - C20（分别含有C18和C20长链碱基）与培养的大鼠小脑颗粒细胞的结合特性进行了研究。两种神经节苷脂在相同程度上识别并与之结合，形成一种结合形式，即对胰蛋白酶敏感的形式。这种形式可通过用胰蛋白酶处理而去除。两种神经节苷脂都与细胞稳定结合，成为细胞膜的组成成分。尽管两种神经节苷脂进入细胞的量相似，随后被代谢，但两种神经节苷脂的结合时间进程不同：在神经节苷脂与细胞孵育15小时后，插入细胞膜的GM1 - C18的量比GM1 - C20高2.43倍。
