控制玉米黑粉菌重组的REC2基因的分离。
Isolation of the REC2 gene controlling recombination in Ustilago maydis.
作者信息
Bauchwitz R, Holloman W K
机构信息
Hearst Microbiology Research Center, Cornell University Medical College, New York, NY 10021.
出版信息
Gene. 1990 Dec 15;96(2):285-8. doi: 10.1016/0378-1119(90)90265-s.
PMID:2269439
Abstract
The rec2 mutant of Ustilago maydis is defective in DNA repair, induced allelic recombination, and meiosis. We cloned the REC2 gene by complementing the radiation-sensitive phenotype of the mutant using a genomic DNA library. The transcript of the REC2 gene was identified as a 2.8-kb damage-inducible RNA.
摘要
玉米黑粉菌的rec2突变体在DNA修复、诱导等位基因重组和减数分裂方面存在缺陷。我们通过使用基因组DNA文库互补该突变体的辐射敏感表型来克隆REC2基因。REC2基因的转录本被鉴定为一种2.8 kb的损伤诱导RNA。
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