Kuntz L, Pflumio F, Velin D, Loor F
Laboratoire d'Immunologie, Université Louis Pasteur, Strasbourg, France.
Immunology. 1990 Nov;71(3):341-6.
Homozygous C57BL/6 nude, beige mice (B6 nu,bg) were used as recipients for the transfer of lymphoid cells from autoimmune homozygous B6 'viable motheaten' mice (B6 mev) and from either normal B6 mice (B6 wild) or B6 bg mice as controls. Surprisingly, the mev cell grafts prolonged survival of these short-living doubly immunodeficient recipients. Although the [mev----nu,bg] chimeras did not develop the mev external necrosis phenotype, they showed a hyperglobulinaemia and a significant increase of their anti-single-stranded DNA (ssDNA) antibody titres, compared to control chimeras ([bg----nu,bg] and [wild----nu,bg]). However, this hyperglobulinaemia was quite different from the mev-type hyperglobulinaemia, with poor contribution of the IgM isotype. Moreover, the anti-ssDNA antibodies were more distributed among the various Ig classes than the anti-ssDNA antibodies of the mev homozygous mice. Though the adoptive transfer of some mev-type humoral autoimmunity symptoms were achieved in this chimera model, the recipient mice did not suffer from the several other features of the mev syndrom, such as the severe pathology and the extremely high IgM serological levels.
纯合子C57BL/6裸鼠、米色小鼠（B6 nu,bg）被用作受体，用于移植来自自身免疫性纯合子B6“活的吞噬缺陷型”小鼠（B6 mev）以及正常B6小鼠（B6野生型）或B6 bg小鼠作为对照的淋巴细胞。令人惊讶的是，mev细胞移植延长了这些寿命短暂的双重免疫缺陷受体的存活时间。尽管[mev----nu,bg]嵌合体没有出现mev外部坏死表型，但与对照嵌合体（[bg----nu,bg]和[野生型----nu,bg]）相比，它们表现出高球蛋白血症，并且其抗单链DNA（ssDNA）抗体滴度显著增加。然而，这种高球蛋白血症与mev型高球蛋白血症有很大不同，IgM同种型的贡献较小。此外，与mev纯合小鼠的抗ssDNA抗体相比，抗ssDNA抗体在各种Ig类别中的分布更为广泛。尽管在此嵌合体模型中实现了一些mev型体液自身免疫症状的过继转移，但受体小鼠并未出现mev综合征的其他几种特征，如严重的病理学表现和极高的IgM血清学水平。
