Environmental Healthcare Unit, Centre for Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
Biofouling. 2012;28(6):563-9. doi: 10.1080/08927014.2012.698387.
Doped diamond-like carbon (DLC) coatings offer potential antifouling surfaces against microbial and protein attachment. In particular, stainless steel surgical instruments are subject to tissue protein and resilient prion protein attachment, making decontamination methods used in sterile service departments ineffective, potentially increasing the risk of iatrogenic Creutzfeldt-Jakob disease during surgical procedures. This study examined the adsorption of proteins and prion-associated amyloid to doped DLC surfaces and the efficacy of commercial cleaning chemistries applied to these spiked surfaces, compared to titanium nitride coating and stainless steel. Surfaces inoculated with ME7-infected brain homogenate were visualised using SYPRO Ruby/Thioflavin T staining and modified epi-fluorescence microscopy before and after cleaning. Reduced protein and prion amyloid contamination was observed on the modified surfaces and subsequent decontamination efficacy improved. This highlights the potential for a new generation of coatings for surgical instruments to reduce the risk of iatrogenic CJD infection.
掺杂类金刚石碳（DLC）涂层提供了具有抗微生物和蛋白质附着潜力的防污表面。特别是不锈钢外科器械容易附着组织蛋白和有弹性的朊病毒蛋白，使无菌服务部门使用的去污方法无效，这可能会增加手术过程中医源性克雅氏病的风险。本研究考察了蛋白质和朊病毒相关淀粉样蛋白在掺杂 DLC 表面上的吸附，以及商业清洁化学品对这些污染表面的有效性，与氮化钛涂层和不锈钢进行了比较。用 SYPRO Ruby/硫黄素 T 染色和改良的 epi-荧光显微镜在接种 ME7 感染的脑匀浆前后观察到污染表面的蛋白质和朊病毒淀粉样蛋白减少，随后的去污效果得到改善。这凸显了新一代外科器械涂层的潜力，可降低医源性 CJD 感染的风险。
