Wine Research Centre, University of Nova Gorica, Vipavska c. 11c, Ajdovščina, Slovenia.
Nat Prod Res. 2013 Apr;27(7):654-64. doi: 10.1080/14786419.2012.688046. Epub 2012 Jun 13.
The foliar anthocyanin profiles of two amphibious plants, Nesaea crassicaulis and Lobelia cardinalis were analysed for the first time. N. crassicaulis produced very simple anthocyanins, achieving the highest concentrations when grown submerged. In contrast, L. cardinalis produced leaves with a high content of very complex, acylated anthocyanins, especially when growing emergent. Anthocyanins were separated by high performance liquid chromatography. Nesaea crassicaulis anthocyanins were identified according to their fragment mass spectra and ultra-visible-violet spectral characteristics and 1D and 2D NMR spectra as -3,5-di-O-β-glucosides of delphinidin, cyanidin, petunidin, malvidin and peonidin as well as cyanidine and peonidin-3-O-β-glucoside. In L. cardinalis cyanidin-3-O-[6-O-(4-O-E-p-coumaroyl-O-α-rhamnopyranosyl)-β-glucopyrano]-5-O-β-glucopyranoside was the major anthocyanin and contributed more than 98% of total anthocyanin content. The remaining 2% was made up by cyanidin-3-O-[6-O-(4-O-E-caffeoyl-O-α-rhamnopyranosyl)-β-glucopyrano]-5-O-β-glucopyranoside and pelargonidin-3-O-[6-O-(4-O-E-p-coumaroyl-O-α-rhamnopyranosyl)-β-glucopyrano]-5-O-β-glucopyranoside.
首次分析了两种两栖植物，Nesaea crassicaulis 和 Lobelia cardinalis 的叶类花色素苷图谱。N. crassicaulis 产生非常简单的花色素苷，在浸没生长时达到最高浓度。相比之下，L. cardinalis 产生的叶子含有高含量的非常复杂的酰化花色素苷，尤其是在长出水面时。花色素苷通过高效液相色谱法分离。根据碎片质谱、超可见紫外线光谱特征以及 1D 和 2D NMR 光谱，Nesaea crassicaulis 花色素苷被鉴定为飞燕草素、矢车菊素、锦葵素、矢车菊素和锦葵素的-3,5-二-O-β-葡萄糖苷，以及矢车菊素和锦葵素-3-O-β-葡萄糖苷。在 L. cardinalis 中，氰花翠素-3-O-[6-O-(4-O-E-对香豆酰-O-α-鼠李吡喃糖苷基)-β-吡喃葡萄糖基]-5-O-β-吡喃葡萄糖苷是主要的花色素苷，占总花色素苷含量的 98%以上。其余 2%由氰花翠素-3-O-[6-O-(4-O-E-咖啡酰-O-α-鼠李吡喃糖苷基)-β-吡喃葡萄糖基]-5-O-β-吡喃葡萄糖苷和矢车菊素-3-O-[6-O-(4-O-E-对香豆酰-O-α-鼠李吡喃糖苷基)-β-吡喃葡萄糖基]-5-O-β-吡喃葡萄糖苷组成。
