Kuskoski E Marta, Vega José M, Rios José J, Fett Roseane, Troncoso Ana M, Asuero Agustin G
Department of Analytical Chemistry, Department of Pharmaceutical and Organic Chemistry, Food Science and Toxicology, Faculty of Pharmacy, The University of Seville, 41012 Seville, Spain.
J Agric Food Chem. 2003 Aug 27;51(18):5450-4. doi: 10.1021/jf030014z.
Anthocyanin pigments in the berries of baguaçu (Eugenia umbelliflora Berg), a tropical fruit from Brazil, were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by Amberlite XAD-7 open-column chromatography. Six major anthocyanins were isolated by preparative HPLC, and their chemical structures were identified by spectroscopic methods (TLC, UV-vis, MS, and (1)H NMR). Delphinidin 3-O-beta-glucopyranoside, cyanidin 3-O-beta-glucopyranoside, petunidin 3-O-beta-glucopyranoside, pelargonidin 3-O-beta-glucopyranoside, peonidin 3-O-beta-glucopyranoside, and malvidin 3-O-beta-glucopyranoside were identified. On the basis of chromatographic data the total anthocyanin content was 342 mg/100 g of fresh baguaçu berries. Therefore, the concomitant presence of six anthocyanins in a single plant species makes this product promising as a new pigment source.
巴西热带水果八卦果（Eugenia umbelliflora Berg）浆果中的花青素色素用0.1%盐酸乙醇溶液提取，粗花青素提取物通过Amberlite XAD-7开放柱色谱法进行纯化。通过制备型高效液相色谱法分离出六种主要花青素，并通过光谱方法（薄层色谱法、紫外可见光谱法、质谱法和氢核磁共振波谱法）鉴定其化学结构。鉴定出了矢车菊素3 - O - β - 吡喃葡萄糖苷、花青素3 - O - β - 吡喃葡萄糖苷、矮牵牛素3 - O - β - 吡喃葡萄糖苷、天竺葵素3 - O - β - 吡喃葡萄糖苷、芍药色素3 - O - β - 吡喃葡萄糖苷和锦葵色素3 - O - β - 吡喃葡萄糖苷。根据色谱数据，新鲜八卦果浆果中总花青素含量为342毫克/100克。因此，单一植物物种中同时存在六种花青素使得该产品有望成为一种新的色素来源。
