Polymer Department, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, China.
J Biomed Mater Res A. 2012 Nov;100(11):2897-904. doi: 10.1002/jbm.a.34226. Epub 2012 Jun 14.
Control of cellular responses is crucial for the use of electrospun membranes in biomedical applications, including tissue engineering or biomedical devices. However, it is still unclear whether adhesion and proliferation of fibroblasts is stimulated or inhibited on polyethylene glycol (PEG)-modified electrospun membranes. In this study, poly(L-lactide-co-glycolide) (PLLGA)-PEG copolymer and pure PEG were blended with PLLGA, and then electrospun onto nonwoven membranes. The effects of blending of PLLGA-PEG or pure PEG on the adsorption of proteins, and further on the adhesion and proliferation of L929 fibroblasts on the electrospun membranes were investigated. Addition of PLLGA-PEG or PEG significantly improved the hydrophilicity of the electrospun membranes. Pure PEG had no obvious effects on the growth of L929 fibroblasts; in contrast, PLLGA-PEG significantly inhibited the adsorption of proteins and the proliferations of the cells on the electrospun membranes. In response to diminished protein adsorption, mRNA expression of genes related to cell adhesion and migration was up-regulated. The limited effects of pure PEG were probably caused by its preferential dissolution, whereas membrane-confined PLLGA-PEG displayed excellent performance on the inhibition of protein adsorption and cell proliferation.
细胞反应的控制对于将电纺膜应用于生物医学领域至关重要，包括组织工程或生物医学设备。然而，对于聚乙二醇（PEG）修饰的电纺膜是否能刺激或抑制成纤维细胞的黏附和增殖，目前仍不清楚。在这项研究中，聚（L-丙交酯-共-乙交酯）（PLLGA）-PEG 共聚物和纯 PEG 与 PLLGA 共混，并通过静电纺丝制成无纺膜。研究了 PLLGA-PEG 或纯 PEG 的共混对蛋白质吸附的影响，以及对 L929 成纤维细胞在电纺膜上黏附和增殖的影响。添加 PLLGA-PEG 或 PEG 显著提高了电纺膜的亲水性。纯 PEG 对 L929 成纤维细胞的生长没有明显影响；相比之下，PLLGA-PEG 显著抑制了蛋白质的吸附和细胞在电纺膜上的增殖。为了减少蛋白质的吸附，与细胞黏附和迁移相关的基因的 mRNA 表达上调。纯 PEG 的有限作用可能是由于其优先溶解所致，而膜限域的 PLLGA-PEG 在抑制蛋白质吸附和细胞增殖方面表现出优异的性能。
