Acharya Chitrangada, Dutta Anindya, Kundu S C
Department of Biotechnology, Indian Institute of Technology Kharagpur-721302, India.
J Biomater Appl. 2009 May;23(6):497-517. doi: 10.1177/0885328208094261. Epub 2008 Sep 18.
This study reports the effects of treatment with various concentrations of organic solvents for varying time points on matrices of fibroin, a silk protein isolated from the mulberry silkworm, Bombyx mori, which in native form has been extensively used in tissue engineering. Treatment of pure fibroin as well as polyethylene glycol- blended films with 90% organic solvent for 60 min induces optimal surface hydrophobicity and maximum conversion of the secondary structure from random coil to beta sheet. Long-term cell viability studies reveal that methanol and isopropanol-treated pure and blended films support cell adhesion, proliferation, and viability.
本研究报告了用不同浓度的有机溶剂在不同时间点处理丝素蛋白基质的效果，丝素蛋白是从家蚕中分离出的一种丝蛋白，其天然形式已广泛应用于组织工程。用90%的有机溶剂处理纯丝素蛋白以及聚乙二醇共混膜60分钟，可诱导出最佳的表面疏水性，并使二级结构从无规卷曲最大程度地转变为β折叠。长期细胞活力研究表明，甲醇和异丙醇处理的纯膜和共混膜能够支持细胞黏附、增殖和存活。
