Discipline of Periodontics, Faculty of Dentistry, National University of Singapore, Singapore 119083, Singapore.
J Biomed Mater Res A. 2012 Nov;100(11):2970-8. doi: 10.1002/jbm.a.34244. Epub 2012 Jun 14.
Proper coordination of local signal to harmonize mitogenesis and osteogenic differentiation is one of the prerequisites to optimize dentoalveolar regeneration. In the study, we purpose to fabricate controlled-release microspheres encapsulating platelet-derived growth factor (PDGF) and simvastatin by coaxial electrohydrodynamic atomization. The microspheres demonstrated a distinct core and shell structure encapsulating PDGF and simvastatin respectively, and the encapsulation efficiency was 82.45-92.16% in-core and 51.37-71.34% in-shell. Sequential release of PDGF and simvastatin was seen in simvastatin-in-core and PDGF-in-shell (SP) design, and simultaneous release was achieved in PDGF-in-core and simvastatin-in-shell (PS) design. All microspheres demonstrated acceptable biocompatibility in vivo, with increased proliferation, reduced apoptosis, and reduced inflammation while PDGF or simvastatin was encapsulated. The PS design significantly reduced apoptosis than control, whereby significant and persistent enhanced proliferation was noted in SP group. The thickness of fibrotic capsules surrounding microspheres significantly reduced in both SP and PS group at day 14. The finding demonstrates that synergism of PDGF and simvastatin favored biocompatibility. Further investigations will aim on confirming the regenerative effect of SP and PS microspheres in a more clinically relevant model.
实现局部信号的适当协调以协调有丝分裂和成骨分化是优化牙牙槽再生的前提之一。在这项研究中，我们旨在通过同轴电动力学雾化来制备包封血小板衍生生长因子（PDGF）和辛伐他汀的控释微球。微球显示出明显的核壳结构，分别包封 PDGF 和辛伐他汀，包封效率分别为 82.45-92.16%的核内和 51.37-71.34%的壳内。在辛伐他汀核内和 PDGF 壳内（SP）设计中观察到 PDGF 和辛伐他汀的顺序释放，在 PDGF 核内和辛伐他汀壳内（PS）设计中实现了同时释放。所有微球在体内均表现出良好的生物相容性，包封 PDGF 或辛伐他汀可增加细胞增殖，减少细胞凋亡和炎症。PS 设计比对照组显著减少细胞凋亡，而 SP 组则显著且持续地增强细胞增殖。在第 14 天时，微球周围纤维囊的厚度在 SP 和 PS 组均显著减少。这些发现表明 PDGF 和辛伐他汀的协同作用有利于生物相容性。进一步的研究将旨在在更具临床相关性的模型中证实 SP 和 PS 微球的再生效果。
