CNRS UMR 7288, Developmental Biology Institute of Marseille Luminy (IBDML), Marseille, France.
J Anat. 2012 Sep;221(3):279-83. doi: 10.1111/j.1469-7580.2012.01529.x. Epub 2012 Jun 15.
Two-photon microscopy (2PM) has become a gold standard for deep-tissue observations in the living animal as well as on thick samples. Using 2PM, the endofluorescence properties of biomolecules have shown an interesting potential for the imaging of tissues without any staining. In this short communication, we report a method to observe the different layers of mouse small intestine explants with subcellular resolution and without any staining or clearing. This method allows rapid observations of samples with little to no preparation thanks to the endofluorescence properties of biomolecules such as NAD(P)H or flavins and second-harmonic generation. Finally, we show different three-dimensional reconstructions of the mouse small intestine anatomy obtained with this approach to show the potential of this method in morphological studies.
双光子显微镜（2PM）已成为活体动物以及厚样本中深层组织观察的金标准。使用 2PM，生物分子的内荧光特性显示出了一种无需任何染色即可对组织进行成像的有趣潜力。在本简讯中，我们报告了一种方法，可以在亚细胞分辨率下观察无任何染色或清除的小鼠小肠外植体的不同层，这种方法允许对具有少量或无需准备的样品进行快速观察，这要归功于生物分子如 NAD(P)H 或黄素的内荧光特性和二次谐波产生。最后，我们展示了使用这种方法获得的小鼠小肠解剖结构的不同三维重建，以显示该方法在形态学研究中的潜力。
