Represa A, Deloulme J C, Sensenbrenner M, Ben-Ari Y, Baudier J
INSERM Unite 29, Paris, France.
J Neurosci. 1990 Dec;10(12):3782-92. doi: 10.1523/JNEUROSCI.10-12-03782.1990.
The developmental expression and the cellular localization of neurogranin (formerly designated p17), a brain-specific protein kinase C (PKC) substrate, were investigated. The developmental expression of neurogranin was studied by immunoblotting of rat brain and neuronal cell-culture extracts using neurogranin polyclonal antibodies. Neurogranin synthesis was found to be developmentally regulated, with no expression in the embryonic and neonatal period and an abrupt increase between 2 and 3 weeks of age. By immunohistochemistry, neurogranin was found essentially in the adult rat telencephalon, specifically located in the cell bodies and dendritic processes of neurons of the cerebral cortex, hippocampus, striatum, and a few other discreet areas. Neurogranin immunoreactivity was nearly absent in the thalamus, cerebellum, and brain stem. The late developmental expression and the dendritic localization of neurogranin in neurons are 2 features that also characterize the type I PKC isozyme. The specific localization of the protein in integrative areas of the rat brain suggests a highly specialized function of neurogranin in the CNS. A possible role for neurogranin in the transduction of the PKC activation signals at the postsynaptic level is suggested.
研究了一种脑特异性蛋白激酶C（PKC）底物——神经颗粒素（以前称为p17）的发育表达及细胞定位。利用神经颗粒素多克隆抗体，通过对大鼠脑和神经元细胞培养提取物进行免疫印迹，研究了神经颗粒素的发育表达。发现神经颗粒素的合成受发育调控，在胚胎期和新生期无表达，在2至3周龄时急剧增加。通过免疫组织化学方法发现，神经颗粒素主要存在于成年大鼠端脑，特别定位于大脑皮层、海马体、纹状体及其他一些离散区域的神经元的细胞体和树突状突起中。在丘脑、小脑和脑干中几乎不存在神经颗粒素免疫反应性。神经颗粒素在神经元中的晚期发育表达和树突定位是I型PKC同工酶也具有的两个特征。该蛋白在大鼠脑整合区域的特异性定位表明神经颗粒素在中枢神经系统中具有高度专业化的功能。提示神经颗粒素在突触后水平转导PKC激活信号中可能发挥作用。
