Peng Junming, Shi Xiaojun, Chen Dingnan, Liang Zhongkun, Lan Kaijian, Gao Jimin, Tan Wanlong
Department of Urinary Surgery, Southern Medical University, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Jun;32(6):807-11.
To develop a novel vaccine by immobilizing interleukin-21 (IL-21) on the surface of MB49 cells and evaluate its effect in inducing specific cytotoxic T lymphocytes (CTLs) and antitumor immunity in a mouse model of subcutaneous metastatic bladder cancer.
SA-IL-21 was immobilized on the surface of 30% ethanol-fixed MB49 cells to prepare the cell vaccine. C57BL/6 mice with subcutaneous implantation of MB49 bladder cancer cells were randomized into 5 groups to receive treatments with IL-21/MB49 vaccine, soluble IL-21, GFP surface-modified MB49 cells, ethanol-fixed MB49 cells, or PBS. The tumor growth and CTL were examined to assess the antitumor efficacy of the vaccine.
IL-21 surface-modified MB49 cell vaccine significantly inhibited the tumor growth and generated a long-lasting memory response (P<0.05). At the same effector-target (E:T) ratio, the specific CTLs induced by IL-21/MB49 vaccine showed the most potent cytotoxicity against MB49 cells (P<0.05).
With the protein-anchor technique, IL-21 can be efficiently immobilized on the surface of MB49 cells to prepare IL-21/MB49 cells vaccine. The novel vaccine can maintain its biological activity and significantly enhance the cytotoxicity of CTLs against bladder cancer cells.
通过将白细胞介素-21（IL-21）固定在MB49细胞表面来开发一种新型疫苗，并在皮下转移性膀胱癌小鼠模型中评估其诱导特异性细胞毒性T淋巴细胞（CTL）和抗肿瘤免疫的效果。
将SA-IL-21固定在30%乙醇固定的MB49细胞表面以制备细胞疫苗。将皮下植入MB49膀胱癌细胞的C57BL/6小鼠随机分为5组，分别接受IL-21/MB49疫苗、可溶性IL-21、绿色荧光蛋白表面修饰的MB49细胞、乙醇固定的MB49细胞或PBS治疗。检测肿瘤生长和CTL以评估疫苗的抗肿瘤疗效。
IL-21表面修饰的MB49细胞疫苗显著抑制肿瘤生长并产生持久的记忆反应（P<0.05）。在相同的效应细胞与靶细胞（E:T）比例下，IL-21/MB49疫苗诱导的特异性CTL对MB49细胞显示出最强的细胞毒性（P<0.05）。
利用蛋白质锚定技术，可将IL-21有效固定在MB49细胞表面以制备IL-21/MB49细胞疫苗。该新型疫苗可保持其生物活性，并显著增强CTL对膀胱癌细胞的细胞毒性。
