State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
Dalton Trans. 2012 Jul 28;41(28):8723-8. doi: 10.1039/c2dt30125c. Epub 2012 Jun 14.
In the present study, we report new water-soluble cell fluorescence imaging and contrast agents that are based on DTPA-AMC(7-amino-4-methyl coumarin)-Eu(3+) and DTPA-AMC(7-amino-4-methyl coumarin)-Gd(3+) compounds conjugated to Fe(3)O(4) NPs via a PEG-NH(2) linker. The novel Fe(3)O(4) NP-conjugates present two main advantages for cell fluorescence labelling: water solubility and targeting ability. The in vitro experiments demonstrate that water-soluble Fe(3)O(4) NPs-DBI-PEG-NH-DTPA-AMC(7-amino-4-methyl coumarin)-Eu(3+) has excellent cell permeating activity. Moreover, the relaxation rate test of Fe(3)O(4) NPs-DBI-PEG-NH-DTPA-AMC(7-amino-4-methyl coumarin)-Gd(3+) shows a higher T1 relaxation effect than traditional DTPA-Gd(3+) MRI agents. According to in vivo liver MRI experiments, better contrast of the liver was achieved after addition of Fe(3)O(4) NPs-DBI-PEG-NH-DTPA-AMC(7-amino-4-methyl coumarin)-Gd(3+). The results will provide a significant guide for researchers exploring the biomedical applications of superparamagnetic Fe(3)O(4) NPs.
在本研究中，我们报道了新的基于 DTPA-AMC（7-氨基-4-甲基香豆素）-Eu（3+）和 DTPA-AMC（7-氨基-4-甲基香豆素）-Gd（3+）化合物的水溶性细胞荧光成像和造影剂，这些化合物通过 PEG-NH2 接头连接到 Fe3O4 NPs 上。新型 Fe3O4 NP 缀合物为细胞荧光标记提供了两个主要优势：水溶性和靶向能力。体外实验表明，水溶性 Fe3O4 NPs-DBI-PEG-NH-DTPA-AMC（7-氨基-4-甲基香豆素）-Eu（3+）具有出色的细胞穿透活性。此外，Fe3O4 NPs-DBI-PEG-NH-DTPA-AMC（7-氨基-4-甲基香豆素）-Gd（3+）的弛豫率测试表明，其 T1 弛豫效应高于传统的 DTPA-Gd（3+）MRI 造影剂。根据体内肝脏 MRI 实验，添加 Fe3O4 NPs-DBI-PEG-NH-DTPA-AMC（7-氨基-4-甲基香豆素）-Gd（3+）后，肝脏的对比效果更好。这些结果将为研究人员探索超顺磁性 Fe3O4 NPs 的生物医学应用提供重要指导。
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