PNA/DNA 杂合体 G-四链体形成对富含 G 序列的人基因组的特异性识别和切割。
G-rich sequence-specific recognition and scission of human genome by PNA/DNA hybrid G-quadruplex formation.
机构信息
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
出版信息
Angew Chem Int Ed Engl. 2012 Jul 16;51(29):7198-202. doi: 10.1002/anie.201201176. Epub 2012 Jun 14.
PMID:22700182
Abstract
Hole in one: A single peptide nucleic acid (PNA) effectively targets the G-rich region in double-stranded DNA through formation of a PNA/DNA hybrid G-quadruplex. Only one target site in the whole human genome was selectively cleaved by the hybrid G-quadruplex. Such site-selective scission of DNA is central to gene manipulation for molecular biology, biotechnology, and therapy.
摘要
单链寡聚体核酸（PNA）与 DNA 形成杂交 G-四链体，能有效靶向双链 DNA 中的富含鸟嘌呤区。整个人类基因组中只有一个靶位被杂交 G-四链体选择性切割。这种 DNA 的位点选择性切割是分子生物学、生物技术和治疗中基因操作的核心。
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