Medical Entomology Unit/WHO Collaborating Centre for Ecology, Taxonomy and Control of Vectors of Malaria, Filariasis and Dengue, Institute for Medical Research, Jalan Pahang, 50588, Kuala Lumpur, Malaysia,
Transgenic Res. 2013 Feb;22(1):47-57. doi: 10.1007/s11248-012-9625-z. Epub 2012 Jun 15.
We conducted the world's first experiments under semi-field conditions (ACL-2 field house) to assess the mating competitiveness of genetically sterile RIDL male mosquitoes (513A strain). The field house is a state-of-the-art, fully-contained trial facility, simulating the living space for a household of 2-4 people in Peninsular Malaysia. Ten genetically sterile RIDL male A. aegypti mosquitoes competed with ten wild type males inside this field house to mate with ten wild type females. Hatched larvae from mated females were screened under a fluorescent microscope for genetic markers to determine if they were fathered by RIDL male or wild type male, and all results were cross-checked by PCR. Two such experiments were conducted, each repeated sufficient number of times. All strains were on a Malaysian lab strain background for the first experiment, while the RIDL males alone were on a recently-colonised Mexican strain background for the second experiment. A total of 52 % of the matings were with RIDL males in the first experiment, while 45 % of the matings were with RIDL (Mexican) males in the second experiment. Statistically, this is not significantly different from 50 % of the matings expected to take place with RIDL males if the latter were as competitive as that of the wild type males. This shows that A. aegypti RIDL-513A has excellent mating competitiveness under semi-field conditions, verifying earlier trends obtained in small lab cages. We also observed high mating compatibility between recently-colonised Mexican RIDL males and lab-reared Malaysian wild type females.
我们在半野外条件（ACL-2 野外屋）下进行了全球首次实验，以评估遗传不育 RIDL 雄蚊（513A 品系）的交配竞争力。该野外屋是一个最先进的、完全封闭的试验设施，模拟了马来西亚半岛一个 2-4 人的家庭居住空间。10 只遗传不育 RIDL 雄埃及伊蚊与 10 只野生型雄蚊在这个野外屋内竞争，与 10 只野生型雌蚊交配。从交配后的雌蚊孵化的幼虫在荧光显微镜下进行遗传标记筛查，以确定它们是由 RIDL 雄蚊还是野生型雄蚊所育，所有结果都通过 PCR 进行交叉检查。进行了两次这样的实验，每个实验都重复了足够多的次数。第一次实验中的所有品系都以马来西亚实验室品系为背景，而第二次实验中的 RIDL 雄蚊单独以最近殖民的墨西哥品系为背景。第一次实验中 RIDL 雄蚊的交配率为 52%，而第二次实验中 RIDL（墨西哥）雄蚊的交配率为 45%。从统计学上讲，如果 RIDL 雄蚊的竞争力与野生型雄蚊相当，那么这与预期的 50%的交配率没有显著差异。这表明 A. aegypti RIDL-513A 在半野外条件下具有出色的交配竞争力，验证了早期在小实验室笼中获得的趋势。我们还观察到最近殖民的墨西哥 RIDL 雄蚊与实验室饲养的马来西亚野生型雌蚊之间具有很高的交配相容性。
