Thomas D D, Donnelly C A, Wood R J, Alphey L S
Department of Zoology, Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
Science. 2000 Mar 31;287(5462):2474-6. doi: 10.1126/science.287.5462.2474.
A major modification to the sterile insect technique is described, in which transgenic insects homozygous for a dominant, repressible, female-specific lethal gene system are used. We demonstrate two methods that give the required genetic characteristics in an otherwise wild-type genetic background. The first system uses a sex-specific promoter or enhancer to drive the expression of a repressible transcription factor, which in turn controls the expression of a toxic gene product. The second system uses non-sex-specific expression of the repressible transcription factor to regulate a selectively lethal gene product. Both methods work efficiently in Drosophila melanogaster, and we expect these principles to be widely applicable to more economically important organisms.
本文描述了对不育昆虫技术的一项重大改进，即使用对显性、可抑制的雌性特异性致死基因系统呈纯合状态的转基因昆虫。我们展示了两种在其他方面为野生型遗传背景下赋予所需遗传特征的方法。第一种系统使用性别特异性启动子或增强子来驱动可抑制转录因子的表达，该转录因子进而控制毒性基因产物的表达。第二种系统使用可抑制转录因子的非性别特异性表达来调节选择性致死基因产物。这两种方法在黑腹果蝇中均有效，我们预计这些原理将广泛应用于更具经济重要性的生物体。
