Department of Biological Production, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Poult Sci. 2012 Jul;91(7):1582-9. doi: 10.3382/ps.2010-01201.
The objective of this study was to investigate the effect of dietary supplementation with 5-aminolevulinic acid (5-ALA) on the immune system, inflammatory response, and growth performance of broiler chickens. The levels of cluster of differentiation 3 (CD3) mRNA in the spleens of chickens gradually increased with dietary 5-ALA concentration, while the expression levels of interleukin (IL)-2 decreased. Mitogen-induced proliferation of splenic mononuclear cells and blood mononuclear cell phagocytosis in chickens fed 0.001 and 0.01% 5-ALA-supplemented diets were significantly greater than in chickens fed a basal diet (control). Plasma thiobarbituric acid reactive substance (TBARS) concentration gradually increased along with 5-ALA supplement concentration. These results provide the first evidence that the use of dietary 0.001 and 0.01% 5-ALA supplementation induces the T-cell immune system via mild oxidative stress in chickens. Three hours after Escherichia coli lipopolysaccharide-induced immune stimulation, the levels of mRNA encoding pro-inflammatory cytokines, such as IL-6 and tumor necrosis factor-like ligand 1A (TL1A), in chickens fed a 0.001% 5-ALA-supplemented diet were significantly lower than those in chickens exposed to other treatments. The plasma caeruloplasmin concentration in chickens fed a 0.001% 5-ALA-supplemented diet was significantly lower than in controls or in chickens fed diets supplemented with other concentrations of 5-ALA 24 h after injection of LPS. In addition, BW at 21 and 50 d of age was significantly higher in chickens fed a 0.001% 5-ALA-supplemented diet than in control chickens. The findings suggest that supplementation of diets with 0.001% 5-ALA could prevent the catabolic changes induced by immunological stimulation. These results show that 5-ALA might be useful as an immunomodulator to stimulate T-cells via mild oxidative stress in growing broiler chickens, thereby improving the growth performance.
本研究旨在探讨 5-氨基酮戊酸（5-ALA）对肉鸡免疫系统、炎症反应和生长性能的影响。随着饲粮 5-ALA 浓度的增加，鸡脾脏中 CD3mRNA 的水平逐渐升高，而白细胞介素（IL）-2 的表达水平降低。饲粮中添加 0.001%和 0.01%5-ALA 可显著增强脾单个核细胞的有丝分裂原刺激增殖和血液单核细胞吞噬作用。随着 5-ALA 补充浓度的增加，血浆硫代巴比妥酸反应物（TBARS）浓度逐渐升高。这些结果首次表明，饲粮中添加 0.001%和 0.01%5-ALA 通过轻度氧化应激诱导鸡的 T 细胞免疫系统。在大肠杆菌脂多糖诱导免疫刺激后 3 小时，饲粮添加 0.001%5-ALA 组鸡促炎细胞因子（如 IL-6 和肿瘤坏死因子样配体 1A（TL1A））的 mRNA 水平显著低于其他处理组。饲粮添加 0.001%5-ALA 组鸡的血浆铜蓝蛋白浓度在 LPS 注射后 24 小时显著低于对照组或其他 5-ALA 浓度添加组。此外，21 和 50 日龄时，饲粮添加 0.001%5-ALA 组鸡的 BW 显著高于对照组。这些结果表明，饲粮添加 0.001%5-ALA 可预防免疫刺激引起的分解代谢变化。这些结果表明，5-ALA 可能作为一种免疫调节剂，通过轻度氧化应激刺激生长肉鸡的 T 细胞，从而改善生长性能。
