Feed Science Institute, Zhejiang University, Hangzhou, China.
Poult Sci. 2012 Jul;91(7):1670-9. doi: 10.3382/ps.2011-02097.
Fatty acid translocase (FAT/CD36) is a transmembrane glycoprotein that plays an important role in transporting long-chain fatty acids. In the current study, a full-length cDNA of FAT/CD36 was first cloned from the intestine of White King pigeon by rapid amplification of cDNA ends (RACE) method. The full-length cDNA of pigeon FAT/CD36 was 2,282 bp, including a 5'-untranslated region of 224 bp, a 3'-untranslated region of 642 bp, and an open reading frame of 1,416 bp encoding a protein of 471 amino acids with the predicted molecular weight of 52.7 kDa. Sequence comparison indicated that FAT/CD36 of pigeon had high identity with other avian FAT/CD36. Using quantitative real-time PCR, expression of FAT/CD36 was the greatest in the duodenum at 28 d posthatch, and in the jejunum, the expression of FAT/CD36 at 14 d posthatch was greater than at 8 d but the same as 28 d posthatch. However, in the ileum, expression of FAT/CD36 peaked at embryonic d 15 and 8 d posthatch. The effects of long-chain fatty acids on pigeon FAT/CD36 and peroxisome proliferator activated receptor γ (PPARγ) mRNA expression were also investigated in vitro. It showed that a low concentration (5 μM) of oleic acid, palmitic acid, and linoleic acid can significantly increase FAT/CD36 and PPARγ mRNA level in pigeon jejunum. However, for linolenic acid or arachidonic acid, the induction of both gene expressions needed a higher concentration (50 μM or 250 μM). Two hundred and 50 μM palmitic acid was shown to suppress FAT/CD36 gene expression. The results suggest that FAT/CD36 may be a representative of intestine development in pigeon, and it could be regulated by long-chain fatty acids via PPARγ pathway.
脂肪酸转运蛋白（FAT/CD36）是一种跨膜糖蛋白，在转运长链脂肪酸方面发挥着重要作用。本研究采用快速扩增 cDNA 末端（RACE）技术，首次从白鸽肠道中克隆得到 FAT/CD36 的全长 cDNA。白鸽 FAT/CD36 的全长 cDNA 为 2282bp，包含 224bp 的 5′非翻译区、642bp 的 3′非翻译区和 1416bp 的开放阅读框，编码一个由 471 个氨基酸组成的蛋白质，预测分子量为 52.7kDa。序列比较表明，白鸽 FAT/CD36 与其他禽类 FAT/CD36 具有高度同源性。采用实时定量 PCR 技术检测到，出壳后 28d 十二指肠 FAT/CD36 表达量最大，出壳后 14d 空肠 FAT/CD36 表达量大于 8d，但与 28d 时相同。然而，回肠 FAT/CD36 的表达在胚胎第 15 天和出壳后 8d 时达到峰值。本研究还体外探讨了长链脂肪酸对白鸽 FAT/CD36 和过氧化物酶体增殖物激活受体γ（PPARγ）mRNA 表达的影响。结果表明，低浓度（5μM）的油酸、棕榈酸和亚油酸可显著增加白鸽空肠 FAT/CD36 和 PPARγ mRNA 水平，而对于亚麻酸或花生四烯酸，这两种基因表达的诱导都需要较高浓度（50μM 或 250μM）。250μM 棕榈酸可抑制 FAT/CD36 基因表达。结果表明，FAT/CD36 可能是白鸽肠道发育的代表，可通过 PPARγ 途径被长链脂肪酸调控。
