Malek M A, Ismail M R, Rafii M Y, Rahman M
Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh 2202, Bangladesh.
ScientificWorldJournal. 2012;2012:416901. doi: 10.1100/2012/416901. Epub 2012 Jun 4.
Brassica napus was synthesized by hybridization between its diploid progenitor species B. rapa and B. oleracea followed by chromosome doubling. Cross with B. rapa as a female parent was only successful. Among three colchicine treatments (0.10, 0.15, and 0.20%), 0.15% gave the highest success (86%) of chromosome doubling in the hybrids (AC; 2n = 19). Synthetic B. napus (AACC, 2n = 38) was identified with bigger petals, fertile pollens and seed setting. Synthetic B. napus had increased growth over parents and exhibited wider ranges with higher coefficients of variations than parents for morphological and yield contributing characters, and yield per plant. Siliqua length as well as beak length in synthetic B. napus was longer than those of the parents. Number of seeds per siliqua, 1000-seed weight and seed yield per plant in synthetic B. napus were higher than those of the parents. Although flowering time in synthetic B. napus was earlier than both parents, however the days to maturity was little higher over early maturing B. rapa parent. The synthesized B. napus has great potential to produce higher seed yield. Further screening and evaluation is needed for selection of desirable genotypes having improved yield contributing characters and higher seed yield.
甘蓝型油菜是由其二倍体祖先物种白菜型油菜和甘蓝杂交后染色体加倍而成。以白菜型油菜为母本进行杂交仅获得成功。在三种秋水仙碱处理(0.10%、0.15%和0.20%)中,0.15%处理使杂种(AC;2n = 19)的染色体加倍成功率最高(86%)。合成的甘蓝型油菜(AACC,2n = 38)花瓣更大、花粉可育且能结实。合成的甘蓝型油菜比亲本生长更快,在形态和产量构成性状以及单株产量方面,其变异系数比亲本更大,范围更广。合成甘蓝型油菜的角果长度和喙长均比亲本长。合成甘蓝型油菜的每角果粒数、千粒重和单株种子产量均高于亲本。虽然合成甘蓝型油菜的开花时间早于双亲,但成熟期比早熟的白菜型油菜亲本略长。合成的甘蓝型油菜具有高产种子的巨大潜力。需要进一步筛选和评估,以选择具有改良产量构成性状和更高种子产量的理想基因型。