Bezault Etienne, Rognon Xavier, Gharbi Karim, Baroiller Jean-Francois, Chevassus Bernard
UMR 110, Cirad-Ifremer INTREPID, 34398 Montpellier, France.
Int J Evol Biol. 2012;2012:870935. doi: 10.1155/2012/870935. Epub 2012 Jun 4.
The transfer of the genomic resources developed in the Nile tilapia, Oreochromis niloticus, to other Tilapiines sensu lato and African cichlid would provide new possibilities to study this amazing group from genetics, ecology, evolution, aquaculture, and conservation point of view. We tested the cross-species amplification of 32 O. niloticus microsatellite markers in a panel of 15 species from 5 different African cichlid tribes: Oreochromines (Oreochromis, Sarotherodon), Boreotilapiines (Tilapia), Chromidotilapines, Hemichromines, and Haplochromines. Amplification was successfully observed for 29 markers (91%), with a frequency of polymorphic (P(95)) loci per species around 70%. The mean number of alleles per locus and species was 3.2 but varied from 3.7 within Oreochromis species to 1.6 within the nontilapia species. The high level of cross-species amplification and polymorphism of the microsatellite markers tested in this study provides powerful tools for a wide range of molecular genetic studies within tilapia species as well as for other African cichlids.
将尼罗罗非鱼（Oreochromis niloticus）中开发的基因组资源转移到其他广义罗非鱼和非洲丽鱼科鱼类中，将为从遗传学、生态学、进化、水产养殖和保护的角度研究这一神奇的群体提供新的可能性。我们在来自5个不同非洲丽鱼科部落的15个物种的样本中测试了32个尼罗罗非鱼微卫星标记的跨物种扩增： Oreochromines（Oreochromis、Sarotherodon）、Boreotilapiines（Tilapia）、Chromidotilapines、Hemichromines和Haplochromines。29个标记（91%）成功观察到扩增，每个物种的多态性（P(95)）位点频率约为70%。每个位点和物种的等位基因平均数为3.2，但在Oreochromis物种中为3.7，在非罗非鱼物种中为1.6。本研究中测试的微卫星标记的高跨物种扩增水平和多态性为罗非鱼物种以及其他非洲丽鱼科鱼类的广泛分子遗传研究提供了强大的工具。
