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永久遗传资源:维多利亚湖慈鲷鱼,Haplochromis chilotes 的微卫星位点的分离与特性。

PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci in the cichlid fish in Lake Victoria, Haplochromis chilotes.

机构信息

Department of Biology, Faculty of Sciences, Kyushu University, 4-2-1 Ropponmatsu, Chuo-ku, Fukuoka 810-8560, Japan, Department of Marine Bioscience, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164-8639, Japan, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):428-30. doi: 10.1111/j.1471-8286.2007.01981.x.

DOI:10.1111/j.1471-8286.2007.01981.x
PMID:21585811
Abstract

Twelve short tandem repeat markers were successfully isolated from a cichlid, Haplochromis chilotes, in Lake Victoria, and characterized in Haplochromis pyrrhocephalus. The microsatellite regions of these markers were found to have between two and 48 alleles with heterozygosity ranging from 0.07 to 0.97. No loci showed significant departures from the Hardy-Weinberg or linkage equilibrium after the Bonferroni correction (P > 0.05). Cross-species amplification in other cichlids of Lake Victoria, Haplochromis laparogramma, Lithochromis rubripinnis, L. rufus and Haplochromis sp. 'rockkribensis', was successful.

摘要

从维多利亚湖的慈鲷鱼哈氏异唇鱼中成功分离出 12 个短串联重复标记,并在哈氏无须魮中进行了特征描述。这些标记的微卫星区域发现有 2 到 48 个等位基因,杂合度范围从 0.07 到 0.97。经过 Bonferroni 校正(P > 0.05)后,没有任何位点显示出与 Hardy-Weinberg 或连锁平衡的显著偏离。在维多利亚湖的其他慈鲷鱼中,如哈氏无须魮、丽鱼、红腹丽鱼和哈氏异唇鱼 sp. 'rockkribensis',也成功地进行了跨物种扩增。

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