Dedeurwaerder G, Duvivier M, Mvuyenkure S M, Renard M E, Van Hese V, Marchal G, Moreau J M, Legrève A
Université catholique de Louvain, Earth and Life Institute, Phytopathology Croix du Sud 2, L7.05.03, BE-1348 Louvain-la-Neuve.
Commun Agric Appl Biol Sci. 2011;76(4):667-70.
A network of Burkard 7-day spore-recording traps was set up in the Walloon Region in Belgium to monitor the airborne inoculum of wheat pathogens. The relationship between the airborne inoculum of Puccinia striiformis f.sp. tritici, the causal agent of stripe rust, and the disease incidence on plants in untreated plots located near each spore traps was studied during the 2008-2009 season. The presence of airborne inoculum was tested in four locations on tapes collected from the Burkard spore traps from 1 April to 14 June 2009. Total DNA from each fragment of spore trap tape corresponding to 1 day sampling was extracted. P. striiformis f.sp. tritici was quantified by real-time polymerase chain reaction (PCR) assay using specific primers and SYBRGreen. The airborne inoculum of P. striiformis was first detected between 7 and 13 April 2009, depending on the location in the Walloon Region. The first symptoms of stripe rust were observed in the fields between 15 May and 2 June 2009. The onset of the disease symptoms was always preceded by a higher peak of airborne inoculum about 15 days earlier. When P. striiformis f.sp. tritici was detected, the daily quantities of spores, collected from a volume of air of 14.4 m3, fluctuated between 0.23 and 154.66. This study shows that spore traps coupled with real-time PCR could be used to assess the airborne inoculum of P. striiformis in order to understand and predict stripe rust outbreaks.
在比利时瓦隆地区建立了一个由 Burkard 7 天孢子记录捕集器组成的网络，以监测小麦病原体的空气传播接种体。在 2008 - 2009 年生长季，研究了条锈病病原菌条形柄锈菌小麦专化型的空气传播接种体与位于每个孢子捕集器附近未处理地块中植株上的病害发病率之间的关系。于 2009 年 4 月 1 日至 6 月 14 日从 Burkard 孢子捕集器收集的胶带的四个位置检测空气传播接种体的存在。提取与 1 天采样对应的每个孢子捕集器胶带片段的总 DNA。使用特异性引物和 SYBRGreen 通过实时聚合酶链反应（PCR）测定法定量条形柄锈菌小麦专化型。根据瓦隆地区的位置，条形柄锈菌的空气传播接种体于 2009 年 4 月 7 日至 13 日首次被检测到。2009 年 5 月 15 日至 6 月 2 日期间在田间观察到条锈病的最初症状。病害症状的出现总是在大约 15 天前先出现空气传播接种体的更高峰值。当检测到条形柄锈菌小麦专化型时，从 14.4 m³ 的空气体积中收集的孢子日数量在 0.23 至 154.66 之间波动。这项研究表明，结合实时 PCR 的孢子捕集器可用于评估条形柄锈菌的空气传播接种体，以便了解和预测条锈病的爆发。
