Duvivier M, Dedeurwaerder G, Marchal G, Renard M E, Van Hese V, Moreau J M, Legreve A
Plant Protection and Ecotoxicology Unit, Centre Wallon de Recherches Agronomiques, 11 rue du Bordia, BE-5030 Gembloux, Belgium.
Commun Agric Appl Biol Sci. 2010;75(4):635-9.
A network of 10 Burkard 7-day spore-recording traps was set up in the Walloon region in Belgium to monitor the airborne inoculum of wheat pathogens. Three spore traps were used to analyse the distribution of Mycosphaerella graminicola inoculum at the field scale, at 1 m above ground level. Two traps were set up in a wheat field 100 m apart. The third trap was placed 70 m away in a sugar beet field adjacent to the wheat field. Total DNA from each fragment of spore trap tape corresponding to 1 day sampling was extracted and the quantity of M. graminicola was assessed using real-time polymerase chain reaction (PCR) assay. The experiment was conducted from July to October 2009. Positive detections were obtained for between 33 and 36 days, depending on the spore traps. When detected, the daily quantities of cDNA, collected from a volume of 14.4 m3, fluctuated between 4.84E+00 and 6.10E+03. Correlation coefficients higher than 0,82 and no significant differences were observed between the quantities of M. graminicola collected by the three spore traps, indicating that, at 1 m above ground level, the distribution of inoculum can be considered as homogenous at the tested field scale. This study confirms that spore traps coupled with real-time PCR could be used to assess the airborne inoculum of M. graminicola and to understand the development of the disease at this scale.
在比利时瓦隆地区设置了一个由10个Burkard 7天孢子记录捕集器组成的网络，以监测小麦病原体的空气传播接种体。使用三个孢子捕集器分析了小麦壳针孢接种体在田间尺度上离地1米高度处的分布情况。在相距100米的一块小麦田中设置了两个捕集器。第三个捕集器放置在距小麦田70米远的一块相邻甜菜田中。提取了与1天采样相对应的每个孢子捕集器胶带片段的总DNA，并使用实时聚合酶链反应（PCR）测定法评估了小麦壳针孢的数量。该实验于2009年7月至10月进行。根据孢子捕集器的不同，阳性检测结果持续了33至36天。检测到的情况下，从14.4立方米体积中收集的每日cDNA量在4.84E+00至6.10E+03之间波动。三个孢子捕集器收集的小麦壳针孢数量之间的相关系数高于0.82，且未观察到显著差异，这表明在离地1米高度处，在所测试的田间尺度上接种体的分布可视为均匀的。这项研究证实，结合实时PCR的孢子捕集器可用于评估小麦壳针孢的空气传播接种体，并了解该尺度下病害的发展情况。
