Svirshchevskaia E V, Dozmorov I M
Radiobiologiia. 1990 Nov-Dec;30(6):754-9.
The ability of lymphocytes to inhibit proliferation of non-syngeneic stem cells decreases differently after exposure in vivo and in vitro. The causes of the observed differences and the mechanism of radiation impairment of this function under different irradiation conditions have been investigated. Cells exposed in vivo die in the interphase irreversibly. The newly formed lymphocytes start the repair process as late as one month after irradiation. The injury to in vivo exposed cells is severer due to the presence of oxygen in tissues. A definite time interval is needed for the damaging effect of oxygen radicals to be implemented: the effect is maximum as early as 4 h following irradiation. With in vivo exposure under hypoxic conditions the functional activity of lymphocytes is the same as that of lymphocytes irradiated in vitro with the same dose. In vitro irradiation of lymphocytes at a high oxygen content causes a decrease in the functional activity of cells.
淋巴细胞抑制非同基因干细胞增殖的能力在体内和体外暴露后会有不同程度的下降。已对观察到的差异原因以及不同照射条件下该功能的辐射损伤机制进行了研究。体内暴露的细胞在间期不可逆地死亡。新形成的淋巴细胞在照射后一个月才开始修复过程。由于组织中存在氧气，体内暴露细胞的损伤更严重。氧自由基的损伤作用需要一定的时间间隔才能显现：照射后4小时作用最大。在缺氧条件下进行体内暴露时，淋巴细胞的功能活性与相同剂量体外照射的淋巴细胞相同。在高氧含量下对淋巴细胞进行体外照射会导致细胞功能活性下降。
