a Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Nanjing, P. R. China.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(11):1672-7. doi: 10.1080/10934529.2012.687256.
Butachlor has adverse effects on fecundity and disrupts sex hormone homeostasis in adult zebrafish, but the underlying molecular mechanisms are still unclear. In the present study, zebrafish (Danio rerio) embryos were exposed to various concentrations of butachlor from 2 h post-fertilization (hpf) to 30 days post-fertilization (dpf). The transcription of genes involved estrogen receptors (ERα, ERβ1 and ERβ2), vitellogenins (VTG I and II), and cytochrome P450 aromatase (CYP19a) was analyzed by real-time quantitative PCR. The results showed that there was no significant alteration in the expression of VTGI, ERα, ERβ1, ERβ2 and CYP19a after 30 days of butachlor exposure, whereas the transcription of VTG II gene was significantly up-regulated in zebrafish exposed to 100 μg/L butachlor. It is suggested that butachlor may be a weak estrogen, and more endpoints need to be investigated to assess the effects of butachlor on the hypothalamus-pituitary-gonadal axis of zebrafish.
丁草胺对成年斑马鱼的生殖力有不良影响，并扰乱了其性激素的体内平衡，但潜在的分子机制尚不清楚。在本研究中，从受精后 2 小时（hpf）到受精后 30 天（dpf），用不同浓度的丁草胺处理斑马鱼胚胎。通过实时定量 PCR 分析了参与雌激素受体（ERα、ERβ1 和 ERβ2）、卵黄蛋白原（VTG I 和 II）和细胞色素 P450 芳香酶（CYP19a）的基因转录。结果表明，暴露于丁草胺 30 天后，VTGI、ERα、ERβ1、ERβ2 和 CYP19a 的表达没有明显改变，而暴露于 100μg/L 丁草胺的斑马鱼的 VTG II 基因转录显著上调。这表明丁草胺可能是一种弱雌激素，需要进一步研究更多的终点来评估丁草胺对斑马鱼下丘脑-垂体-性腺轴的影响。
