University of Münster, Institute of Inorganic and Analytical Chemistry and NRW Graduate School of Chemistry, Corrensstr. 30, 48149 Münster, Germany.
J Chromatogr A. 2012 Oct 12;1259:16-49. doi: 10.1016/j.chroma.2012.05.066. Epub 2012 May 27.
The coupling of electrochemistry (EC) to different mass spectrometric (MS) techniques in off-line and especially in on-line approaches is a quickly growing research field in analytical chemistry with numerous distinct objectives. Depending on the analytical problem, a separation step can be further integrated according to the instrumental set-up and, most frequently, liquid chromatography (LC) is selected for this purpose. In this review, various scientific areas of application for this EC/(LC/)MS hybrid method are presented and discussed in detail. Therefore, one major division is made between those applications which are already successfully used on a large scale (current state), and those which have shown promising results for future utilization (future perspectives). The reader shall be provided with a thorough overview on the capabilities of the combination of EC/(LC/)MS and the drawbacks which result in further optimization and exploration of this technique. The major topics addressed in this review include the role of EC/(LC/)MS for drug metabolism studies, peptide, protein and DNA (deoxyribonucleic acid) research and quantification strategies. Promising future applications that are presented and evaluated comprise the fields of toxicology and forensics, targeted product synthesis and environmental analysis.
电化学（EC）与不同的质谱（MS）技术在离线和特别是在线方法中的结合是分析化学中一个快速发展的研究领域，具有许多不同的目标。根据分析问题，可以根据仪器设置进一步集成分离步骤，并且最常为此选择液相色谱（LC）。在这篇综述中，详细介绍并讨论了这种 EC/（LC/）MS 混合方法的各种科学应用领域。因此，主要分为已经大规模成功应用的（当前状态）和具有未来应用前景的（未来展望）。应向读者提供有关 EC/（LC/）MS 组合的功能以及导致该技术进一步优化和探索的缺点的全面概述。这篇综述中涉及的主要主题包括 EC/（LC/）MS 在药物代谢研究、肽、蛋白质和 DNA（脱氧核糖核酸）研究以及定量策略中的作用。提出并评估的有前途的未来应用包括毒理学和法医学、靶向产品合成和环境分析领域。
