Division of Gastroenterology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50, Irwon-dong, Gangnam-gu, Seoul 135-710, South Korea.
Clin Res Hepatol Gastroenterol. 2013 Apr;37(2):159-65. doi: 10.1016/j.clinre.2012.04.013. Epub 2012 Jun 15.
An animal model for bile duct stenosis using intraductal thermal injury has not yet been established. The aims of the current study were to develop biliary stenosis in a swine model by inducing intraductal thermal injury using a heat probe or radiofrequency ablation electrode and to investigate an effective and safe energy dose.
Intraluminal thermal injury was applied to the common bile duct with a heat probe in three swines and a radiofrequency ablation electrode in the other three swines by either endoscopic retrograde cholangiography or open laparotomy. Cholangiography and histologic evaluation of common bile duct were taken 2 weeks after thermal injury.
Thermal injury with a heat probe at 25 J for 40 seconds produced a stricture in all three animals. Application of a radiofrequency ablation electrode produced a stricture in two of three animals. An energy dose of 40 W at 80°C for 30 seconds produced biliary stenosis without any complications initially and 2 weeks after thermal injury.
The application of a heat probe and a radiofrequency ablation electrode for intraductal thermal injury resulted in a reproducible animal model of biliary stenosis.
尚未建立胆管狭窄的动物模型，通过胆管内热损伤来实现。本研究的目的是通过使用热探头或射频消融电极在猪模型中诱导胆管内热损伤，从而开发胆道狭窄模型，并研究有效且安全的能量剂量。
通过内镜逆行胰胆管造影术或剖腹手术，将热探头应用于三只猪的胆总管内，用射频消融电极应用于另外三只猪的胆总管内，进行腔内热损伤。热损伤 2 周后进行胆管造影和胆总管组织学评估。
使用热探头，25 J 持续 40 秒，在所有三只动物中均产生了狭窄。应用射频消融电极，在三只动物中有两只产生了狭窄。40 W、80°C、30 秒的能量剂量最初和热损伤 2 周后均未产生并发症，导致了胆管狭窄。
应用热探头和射频消融电极进行胆管内热损伤，可产生可重现的胆管狭窄动物模型。
