Department of Internal Medicine, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, South Korea.
Division of Gastroenterology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul, 06351, South Korea.
Sci Rep. 2023 May 3;13(1):7185. doi: 10.1038/s41598-023-33867-9.
Several in vivo swine models of benign biliary stenosis (BBS) have been recently reported for preclinical studies of novel endoscopic techniques and devices. The aim of this study was to evaluate the efficacy and feasibility of large animal models of BBS by using intraductal radiofrequency ablation (RFA) assisted by guide wire. Six in vivo swine models were made by using an intraductal RFA for cauterization at 10 W, 80 °C, 90 s in the common bile duct (CBD). Endoscopic retrograde cholangiopancreatography (ERCP) was performed with cholangiography and histologic evaluation was done for the common bile duct. Blood tests were examined before, after, and at the final follow-up. Guide wire assisted RFA electrode produced BBS in all (6/6, 100%) animal models without severe complications. Fluoroscopy findings at 2 weeks after intraductal RFA in every model revealed BBS in the common bile duct. In histologic evaluations, fibrosis and chronic inflammatory changes were noted. After the procedure, ALP, GGT, and CRP were elevated and decreased after an appropriate drain. A swine model of BBS is developed by inducing intraductal thermal injury using intraductal RFA assisted by guide wire. This novel technique for inducing BBS in swine is effective and feasible.
几种良性胆道狭窄（BBS）的体内猪模型最近已被报道用于新型内镜技术和设备的临床前研究。本研究的目的是通过使用导丝辅助的腔内射频消融（RFA）来评估 BBS 大动物模型的疗效和可行性。在胆总管（CBD）中以 10 W、80°C、90 s 的功率进行腔内 RFA 烧灼，建立了 6 个体内猪模型。进行内镜逆行胰胆管造影（ERCP）并进行胆管造影，对胆总管进行组织学评估。在术前、术后和最终随访时检查血液检查。导丝辅助 RFA 电极在所有（6/6，100%）动物模型中均产生 BBS，没有严重的并发症。在每个模型的腔内 RFA 后 2 周的透视检查中，均显示胆总管存在 BBS。组织学评估显示纤维化和慢性炎症改变。在该操作后，碱性磷酸酶（ALP）、谷氨酰转肽酶（GGT）和 C 反应蛋白（CRP）升高，适当引流后降低。通过导丝辅助的腔内 RFA 诱导腔内热损伤，建立了 BBS 的猪模型。这种诱导猪 BBS 的新技术是有效且可行的。
